Comparative genome analysis and identification of competitive and cooperative interactions in a polymicrobial disease.
Comparative genome analysis and identification of competitive and cooperative interactions in a polymicrobial disease.
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DOI：
10.1038/ismej.2014.155
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发表时间：
2015-03
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影响因子：
--
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多微生物疾病是由多种细菌组合引起的，不仅会导致轻微疾病，还会导致危及生命的疾病。牙周炎是一种多微生物疾病，牙龈卟啉单胞菌、齿垢密螺旋体和嗜酸坦纳菌被称为“红色复合体”，被认为是牙周炎的病原体。虽然这三个物种之间的分子相互作用可能是牙周炎进展的原因，但相关的遗传机制尚不清楚。在这项研究中，我们发现了新的相互作用的比较基因组分析之间的红色复杂的物种。T.该菌可能攻击牙龈卟啉单胞菌的限制性修饰系统，并可能作为牙龈卟啉单胞菌DNA入侵的防御系统。另一方面，基因缺陷相互补偿的代谢途径时，考虑到所有的三个物种的基因，这表明有三个物种之间的合作关系。这一观点得到以下观察结果的支持：这三个物种中的每一个都有自己的毒力因子，这可能有助于这三个物种的毒力的持久性和表现。在这里，我们提出了牙周炎细菌共生的新机制，这些机制包括竞争和合作的相互作用。我们的研究结果可能有助于阐明牙周炎和其他多种微生物疾病的发病机制。
Polymicrobial diseases are caused by combinations of multiple bacteria, which can lead to not only mild but also life-threatening illnesses. Periodontitis represents a polymicrobial disease; Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia, called ‘the red complex', have been recognized as the causative agents of periodontitis. Although molecular interactions among the three species could be responsible for progression of periodontitis, the relevant genetic mechanisms are unknown. In this study, we uncovered novel interactions in comparative genome analysis among the red complex species. Clustered regularly interspaced short palindromic repeats (CRISPRs) of T. forsythia might attack the restriction modification system of P. gingivalis, and possibly work as a defense system against DNA invasion from P. gingivalis. On the other hand, gene deficiencies were mutually compensated in metabolic pathways when the genes of all the three species were taken into account, suggesting that there are cooperative relationships among the three species. This notion was supported by the observation that each of the three species had its own virulence factors, which might facilitate persistence and manifestations of virulence of the three species. Here, we propose new mechanisms of bacterial symbiosis in periodontitis; these mechanisms consist of competitive and cooperative interactions. Our results might shed light on the pathogenesis of periodontitis and of other polymicrobial diseases.
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影响因子：
3.7
作者：
Ge X;Rodriguez R;Trinh M;Gunsolley J;Xu P
通讯作者：
Xu P
影响因子：
--
作者：
Fuerst, John A;Sagulenko, Evgeny
通讯作者：
Sagulenko, Evgeny
影响因子：
3.5
作者：
BOTTA, GA;RADIN, L;BLASI, G
通讯作者：
BLASI, G
影响因子：
11
作者：
Griffen, Ann L.;Beall, Clifford J.;Leys, Eugene J.
通讯作者：
Leys, Eugene J.
DOI：
10.1099/00221287-137-5-1201
发表时间：
1991-05-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
GHARBIA, SE;SHAH, HN
通讯作者：
SHAH, HN