Oral microbiome of deep and shallow dental pockets in chronic periodontitis.
Oral microbiome of deep and shallow dental pockets in chronic periodontitis.
复制标题
DOI：
10.1371/journal.pone.0065520
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Xu P
中科院分区：
文献类型：
--
作者：
Ge X;Rodriguez R;Trinh M;Gunsolley J;Xu P
我们通过对16 S rRNA基因进行测序，检测了未经治疗的慢性牙周炎患者龈下细菌的生物多样性。该研究的主要目的是比较深层（患病）和浅层（健康）部位的口腔微生物组。第二个目的是评估吸烟，种族和龋齿对这种关系的影响。共招募了来自两个诊所的88名受试者。从每个受试者中采集成对的龈下菌斑样本，一个来自探测部位深度>5 mm（深部位），另一个来自探测部位深度≤ 3 mm（浅部位）。设计了一套通用引物，用于扩增口腔微生物16 S rRNA序列的V4-V6区域。属和种的差异归因于深和浅的网站，通过统计分析，使用两部分模型和错误的发现率。170属中有51属，746种中有200种在浅水区和深水区的丰度存在显著差异。除了以前确定的牙周病相关的细菌物种，其他物种被发现在患病部位显着变化。聚类分析显示，深部和浅层部位之间的微生物组差异受到患者水平效应的影响，如诊所位置，种族和吸烟。诊所位置之间的差异可能受到种族分布的影响，因为所有非裔美国人受试者都在同一家诊所就诊。我们的研究结果表明，微生物组对龋齿和牙周病有影响，这些影响是独立的。
We examined the subgingival bacterial biodiversity in untreated chronic periodontitis patients by sequencing 16S rRNA genes. The primary purpose of the study was to compare the oral microbiome in deep (diseased) and shallow (healthy) sites. A secondary purpose was to evaluate the influences of smoking, race and dental caries on this relationship. A total of 88 subjects from two clinics were recruited. Paired subgingival plaque samples were taken from each subject, one from a probing site depth >5 mm (deep site) and the other from a probing site depth ≤3mm (shallow site). A universal primer set was designed to amplify the V4–V6 region for oral microbial 16S rRNA sequences. Differences in genera and species attributable to deep and shallow sites were determined by statistical analysis using a two-part model and false discovery rate. Fifty-one of 170 genera and 200 of 746 species were found significantly different in abundances between shallow and deep sites. Besides previously identified periodontal disease-associated bacterial species, additional species were found markedly changed in diseased sites. Cluster analysis revealed that the microbiome difference between deep and shallow sites was influenced by patient-level effects such as clinic location, race and smoking. The differences between clinic locations may be influenced by racial distribution, in that all of the African Americans subjects were seen at the same clinic. Our results suggested that there were influences from the microbiome for caries and periodontal disease and these influences are independent.
登录
查看更多内容
影响因子：
6.7
作者：
Axelsson, P;Nyström, B;Lindhe, J
通讯作者：
Lindhe, J
影响因子：
18.6
作者：
Paster, Bruce J.;Olsen, Ingar;Dewhirst, Floyd E.
通讯作者：
Dewhirst, Floyd E.
影响因子：
3.7
作者：
Jumpstart Consortium Human Microbiome Project Data Generation Working Group
通讯作者：
Jumpstart Consortium Human Microbiome Project Data Generation Working Group
影响因子：
3.7
作者：
Liu B;Faller LL;Klitgord N;Mazumdar V;Ghodsi M;Sommer DD;Gibbons TR;Treangen TJ;Chang YC;Li S;Stine OC;Hasturk H;Kasif S;Segrè D;Pop M;Amar S
通讯作者：
Amar S
影响因子：
11
作者：
Griffen, Ann L.;Beall, Clifford J.;Leys, Eugene J.
通讯作者：
Leys, Eugene J.