Prenatal exposure to urban air nanoparticles in mice causes altered neuronal differentiation and depression-like responses.
Prenatal exposure to urban air nanoparticles in mice causes altered neuronal differentiation and depression-like responses.
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DOI：
10.1371/journal.pone.0064128
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Morgan TE
中科院分区：
文献类型：
--
作者：
Davis DA;Bortolato M;Godar SC;Sander TK;Iwata N;Pakbin P;Shih JC;Berhane K;McConnell R;Sioutas C;Finch CE;Morgan TE
新出现的证据表明，怀孕期间过度暴露于交通引起的空气污染可能会增加神经发育改变的脆弱性，这些改变是一系列神经精神疾病的基础。我们提出了一个小鼠模型，产前暴露于城市高速公路纳米颗粒物（nPM）。在先前的研究中，我们开发了一种成年啮齿动物暴露于再雾化的城市nPM的模型，该模型引起炎症性脑反应，改变了神经元神经元的功能。从当地高速公路连续收集nPM一个月，并作为水性悬浮液储存，然后再雾化以在受控剂量和持续时间下暴露于小鼠。这种模式被用于产前nPM对新生儿神经元和成人行为的影响的试点研究。成年C57 BL/6 J雌性小鼠暴露于再雾化nPM（350 µg/m3）或对照过滤环境空气10周（每周暴露3×5小时），包括妊娠和交配前卵母细胞成熟。产前nPM没有改变窝仔数，幼犬体重，或出生后的增长。在体外24小时的新生大脑皮层神经元显示出受损的分化，具有长神经突的第3阶段神经元减少50%，并且相应地在第0和第1阶段有更多的未分化神经元。培养24小时后的神经元数量没有改变产前nPM暴露。向培养物中加入外源性nPM（2 μg/ml），锥体神经元3期分化受损60%。成年男性在尾部悬吊试验中表现出抑郁样反应增加，但与焦虑相关的行为没有增加。这些试点数据表明，产前暴露于nPM可以改变神经元分化与性别特异性的行为后遗症，可能与人类产前暴露于城市车辆气溶胶。
Emerging evidence suggests that excessive exposure to traffic-derived air pollution during pregnancy may increase the vulnerability to neurodevelopmental alterations that underlie a broad array of neuropsychiatric disorders. We present a mouse model for prenatal exposure to urban freeway nanoparticulate matter (nPM). In prior studies, we developed a model for adult rodent exposure to re-aerosolized urban nPM which caused inflammatory brain responses with altered neuronal glutamatergic functions. nPMs are collected continuously for one month from a local freeway and stored as an aqueous suspension, prior to re-aerosolization for exposure of mice under controlled dose and duration. This paradigm was used for a pilot study of prenatal nPM impact on neonatal neurons and adult behaviors. Adult C57BL/6J female mice were exposed to re-aerosolized nPM (350 µg/m3) or control filtered ambient air for 10 weeks (3×5 hour exposures per week), encompassing gestation and oocyte maturation prior to mating. Prenatal nPM did not alter litter size, pup weight, or postnatal growth. Neonatal cerebral cortex neurons at 24 hours in vitro showed impaired differentiation, with 50% reduction of stage 3 neurons with long neurites and correspondingly more undifferentiated neurons at Stages 0 and 1. Neuron number after 24 hours of culture was not altered by prenatal nPM exposure. Addition of exogenous nPM (2 µg/ml) to the cultures impaired pyramidal neuron Stage 3 differentiation by 60%. Adult males showed increased depression-like responses in the tail-suspension test, but not anxiety-related behaviors. These pilot data suggest that prenatal exposure to nPM can alter neuronal differentiation with gender-specific behavioral sequelae that may be relevant to human prenatal exposure to urban vehicular aerosols.
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影响因子：
2.5
作者：
Calderon-Garciduenas, Lilian;Engle, Randall;D'Angiulli, Amedeo
通讯作者：
D'Angiulli, Amedeo
影响因子：
10.6
作者：
Liu, XQ;Gershenfeld, HK
通讯作者：
Gershenfeld, HK
影响因子：
2.9
作者：
BANKER, GA;COWAN, WM
通讯作者：
COWAN, WM
影响因子：
3.5
作者：
Currie, Janet;Neidell, Matthew;Schmieder, Johannes F.
通讯作者：
Schmieder, Johannes F.
DOI：
10.1002/ar.21299
发表时间：
2011-10
影响因子：
2
作者：
Fatemi, S. Hossein;Folsom, Timothy D.;Kneeland, Rachel E.;Liesch, Stephanie B.
通讯作者：
Liesch, Stephanie B.