Reduced miR-29a-3p expression is linked to the cell proliferation and cell migration in gastric cancer.
Reduced miR-29a-3p expression is linked to the cell proliferation and cell migration in gastric cancer.
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miR-29a-3p表达减少与胃癌细胞增殖和细胞迁移有关
DOI：
10.1186/s12957-015-0513-x
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发表时间：
2015-03-12
影响因子：
3.2
通讯作者：
Fan H
中科院分区：
文献类型：
--
作者：
Zhao Z;Wang L;Song W;Cui H;Chen G;Qiao F;Hu J;Zhou R;Fan H
MicroRNAs(MiRNAs)在肿瘤的抑制或致癌过程中发挥着重要作用。MiRNAs在胃癌中的表达模式改变与肿瘤的发生发展密切相关。本研究旨在探讨miR-29a-3p的表达模式及其在胃癌发生中的作用。采用实时定量聚合酶链式反应(qRT-PCR)检测miR-29a-3p在胃癌组织和细胞系中的表达。转染miR-29a-3p模拟物或抑制剂后，分别用CCK-8比色法、创伤愈合实验和Transans-well实验检测细胞的增殖、迁移和侵袭能力。用qRT-PCR和Western印迹法检测CDK2、CDK4、CDK6和细胞周期蛋白D1的表达水平。与相应的非肿瘤组织相比，miR-29a-3p在肿瘤组织中的表达显著下调。体外功能分析表明，miR-29a-3p的增强表达通过降低CDK2、CDK4和CDK6的表达而抑制细胞增殖。伤口愈合和Transwell检测显示miR-29a-3p抑制了胃癌的肿瘤转移。我们的初步结果提示miR-29a-3p的表达改变参与了胃癌的发生发展过程。本研究首次揭示了miR-29a-3p在胃癌发生中的具体作用。本文的在线版本(doi：10.1186/s12957-0150513-x)包含补充材料，授权用户可以使用。
MicroRNAs (miRNAs) play an important role in a tumor-suppressive or oncogenic manner in carcinogenesis. Alteration expression patterns of miRNAs in gastric cancer (GC) are associated with cancer initiation and progression. In the present study, we evaluated miR-29a-3p expression pattern and its function in gastric carcinogenesis. The expression of miR-29a-3p in GC tissue samples and cell lines was detected by quantitative real-time PCR (qRT-PCR). After transfected with miR-29a-3p mimics or inhibitor, the cell proliferation, cell migration, and invasion ability were assessed by CCK-8 assay, wound healing assay, and Trans-well assay, respectively. The level of CDK2, CDK4, CDK6, and CyclinD1 were determined by qRT-PCR and Western blot. Compared with the corresponding non-tumor tissues, miR-29a-3p showed a significant down-regulated expression in tumor tissues. In vitro functional assays demonstrated that enforced miR-29a-3p expression inhibited cell proliferation by reducing the expression of CDK2, CDK4, and CDK6. Wound healing and Transwell assays revealed that miR-29a-3p suppressed tumor metastasis in GC. Our preliminary results suggest that altered expression of miR-29a-3p is involved in gastric cancer process. The present study provides the first insight into the specific role of miR-29a-3p in gastric carcinogenesis. The online version of this article (doi:10.1186/s12957-015-0513-x) contains supplementary material, which is available to authorized users.
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DOI：
10.1084/jem.20090831
发表时间：
2010-03-15
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Han YC;Park CY;Bhagat G;Zhang J;Wang Y;Fan JB;Liu M;Zou Y;Weissman IL;Gu H
通讯作者：
Gu H
影响因子：
50.3
作者：
Wang H;Garzon R;Sun H;Ladner KJ;Singh R;Dahlman J;Cheng A;Hall BM;Qualman SJ;Chandler DS;Croce CM;Guttridge DC
通讯作者：
Guttridge DC
影响因子：
3.1
作者：
Wang, Fang;Sun, Guo-Ping;Ren, Wen-Jun
通讯作者：
Ren, Wen-Jun
影响因子：
8
作者：
Mott, J. L.;Kobayashi, S.;Gores, G. J.
通讯作者：
Gores, G. J.
DOI：
10.1016/j.bbrc.2013.04.010
发表时间：
2013-05-10
影响因子：
3.1
作者：
Wang, Fang;Li, Ting;Fan, Daiming
通讯作者：
Fan, Daiming