TRPV1 channel-mediated bilateral allodynia induced by unilateral masseter muscle inflammation in rats.
TRPV1 channel-mediated bilateral allodynia induced by unilateral masseter muscle inflammation in rats.
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DOI：
10.1186/1744-8069-9-68
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发表时间：
2013-12-30
期刊：
影响因子：
3.3
通讯作者：
Wang K
中科院分区：
文献类型：
--
作者：
Simonic-Kocijan S;Zhao X;Liu W;Wu Y;Uhac I;Wang K
咀嚼肌疼痛是颞下颌关节紊乱病（Temperomandibular disorders，TMD）最突出的症状之一，其病因多样而复杂。颞下颌关节紊乱病的一个常见症状是单侧头面部肌肉疼痛可引起广泛的双侧疼痛感，但其机制尚不清楚。为了探讨单侧咬肌炎症是否会引起双侧异常性疼痛，我们通过单侧注射完全弗氏佐剂（CFA）诱导大鼠咬肌炎症，并使用von Frey麻醉仪在不同时间点测量双侧头部回缩阈值。行为评估后，右和左三叉神经节（TRG）解剖和组织病理学检查和瞬时受体电位香草素1（TRPV 1）mRNA的表达，使用定量实时PCR分析。TRPV 1 mRNA表达的显着增加发生在TRG同侧CFA注射咬肌，而没有显着的改变，TRPV 1发生在对侧TRG。有趣的是，中央注射TRPV 1拮抗剂5-碘树脂毒素到海马显着衰减CFA注射和非CFA注射对侧咬肌的头部撤回反应。我们的研究结果表明，单侧咬肌炎症能够诱导大鼠双侧异常性疼痛。TRPV 1在TRG水平的上调是由于炎症引起的伤害性感受，而咀嚼肌伤害性感受中的对侧伤害性反应行为可能是由中央TRPV 1介导的，这表明涉及更高中心的信息处理改变。
Pain in masticatory muscles is among the most prominent symptoms of temperomandibular disorders (TMDs) that have diverse and complex etiology. A common complaint of TMD is that unilateral pain of craniofacial muscle can cause a widespread of bilateral pain sensation, although the underlying mechanism remains unknown. To investigate whether unilateral inflammation of masseter muscle can cause a bilateral allodynia, we generated masseter muscle inflammation induced by unilateral injection of complete Freund’s adjuvant (CFA) in rats, and measured the bilateral head withdrawal threshold at different time points using a von Frey anesthesiometer. After behavioral assessment, both right and left trigeminal ganglia (TRG) were dissected and examined for histopathology and transient receptor potential vanilloid 1 (TRPV1) mRNA expression using quantitative real-time PCR analysis. A significant increase in TRPV1 mRNA expression occurred in TRG ipsilateral to CFA injected masseter muscle, whereas no significant alteration in TRPV1 occurred in the contralateral TRG. Interestingly, central injection of TRPV1 antagonist 5-iodoresiniferatoxin into the hippocampus significantly attenuated the head withdrawal response of both CFA injected and non-CFA injected contralateral masseter muscle. Our findings show that unilateral inflammation of masseter muscle is capable of inducing bilateral allodynia in rats. Upregulation of TRPV1 at the TRG level is due to nociception caused by inflammation, whereas contralateral nocifensive behavior in masticatory muscle nociception is likely mediated by central TRPV1, pointing to the involvement of altered information processing in higher centers.
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