Bruguierivorax albus gen. nov. sp. nov. Isolated from Mangrove Sediment and Proposal of Bruguierivoracaceae fam. nov.
Bruguierivorax albus gen. nov. sp. nov. Isolated from Mangrove Sediment and Proposal of Bruguierivoracaceae fam. nov.
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白木榄属 (Bruguierivorax albus)
DOI：
10.1007/s00284-020-02311-w
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发表时间：
2021-03
影响因子：
2.6
通讯作者：
Yi X
中科院分区：
文献类型：
--
作者：
Li M;Liu K;Liu Y;Gao C;Yi X
从中国广西省的红树林沉积物中分离出一种新型革兰氏阴性、能动、需氧的杆状细菌，命名为 BGMRC 2031T。最佳生长条件为 28 °C、pH 7.0–8.0、1%（w/v）氯化钠存在。基于16S rRNA基因序列的比对表明，菌株BGMRC 2031T与Sodalis praecaptivus HS1T关系最密切（95.6％，序列相似性），其次是Biostraticola tofi DSM 19580T（95.5％），Sodalisglassinidius DSM 16929T（95.4％）和Brenneria goodwinii FRB141T (94.9%) 序列相似性。基于16S rRNA基因序列的系统发育分析表明，菌株BGMRC 2031T在一个强大的簇中形成了一个独特的分支，并揭示菌株BGMRC 2031T、Biostraticola属和Sodalis属在肠杆菌目中形成了一个新的科级进化枝。该新菌株与 S. praecaptivus HS1T、S. Glossinidius DSM 16929T 和 B. tofi DSM 19580T 的平均核苷酸相似性分别为 74.7%、74.2% 和 73.1%。 BGMRC 2031T 的基因组共享核黄素合成基因簇的存在。菌株BGMRC 2031T的甲基萘醌类化合物为MK-8和Q-8，与Biostraticola属的甲基萘醌类化合物相似。主要脂肪酸 (> 10%) 为 C16:0 (19.9%)、汇总特征 2（iso-C16:1 和/或 C14:0 3-OH，18.10%）、汇总特征 3（C16:1 ω7c 和/或 C16:1 ω6c，15.3%）、C12:0 (13.9%)、C17:0环 (11.4%) 和 C14:0 (10.4%)。主要极性脂质为磷脂酰甲基乙醇胺、磷脂酰甘油、二磷脂酰甘油、磷脂酰肌醇、一种未鉴定的磷脂和一种未知的极性脂质。菌株BGMRC 2031T的G+C含量为55.4%。菌株 BGMRC 2031T 可使秀丽隐杆线虫的平均寿命和最大寿命分别延长 4.5% 和 12.5%。总体而言，本研究结果表明 BGMRC 2031T 是一个新属的新物种，其名称为 Bruguierivorax albus gen.十一月sp。十一月提出，菌株类型指定为BGMRC 2031T（= NBRC 111907T = KCTC 52119T）。此外，还有一个新科，Bruguierivoracaceae科。 11 月，建议容纳 Bruguierivorax 属、Biostraticola 属和 Sodalis 属。本文的在线版本 (10.1007/s00284-020-02311-w) 包含补充材料，可供授权用户使用。
A novel Gram-negative, motile, aerobic rod-shaped bacterium designated BGMRC 2031T was isolated from mangrove sediment collected from Guangxi Province, China. Optimal growth occurred at 28 °C and pH 7.0–8.0 in the presence of 1% (w/v) NaCl. Alignment based on 16S rRNA gene sequences indicated that strain BGMRC 2031T is most closely related to Sodalis praecaptivus HS1T (95.6%, sequence similarity), followed by Biostraticola tofi DSM 19580T (95.5%), Sodalis glossinidius DSM 16929T (95.4%), and Brenneria goodwinii FRB141T (94.9%) sequence similarity. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BGMRC 2031T formed a distinct branch in a robust cluster and revealed that strain BGMRC 2031T, genera Biostraticola and Sodalis, formed a novel family-level clade in the order Enterobacterales. The novel strain showed an average nucleotide similarity of 74.7%, 74.2%, and 73.1% for S. praecaptivus HS1T, S. glossinidius DSM 16929T, and B. tofi DSM 19580T, respectively. The genomes of the BGMRC 2031T shared the presence of a riboflavin synthesis gene cluster. The menaquinones of strain BGMRC 2031T were MK-8 and Q-8, which were similar to those of genus Biostraticola. The major fatty acids (> 10%) were C16:0 (19.9%), summed feature 2 (iso-C16:1 and/or C14:0 3-OH, 18.10%), summed feature 3 (C16:1 ω7c and/or C16:1 ω6c, 15.3%), C12:0 (13.9%), C17:0 cyclo (11.4%), and C14:0 (10.4%). The main polar lipids were phosphatidyl methylethanolamine, phosphatidyl glycerol, diphosphatidyl glycerol, phosphatidyl inositol, one unidentified phospholipid, and one unknown polar lipid. The G+C content of strain BGMRC 2031T was 55.4%. Strain BGMRC 2031T could extend the mean lifespan and maximum lifespan of Caenorhabditis elegans by 4.5% and 12.5%, respectively. Overall, the results of this study indicate that BGMRC 2031T is a novel species in a new genus, for which the name Bruguierivorax albus gen. nov. sp. nov. is proposed, and the type of strain is designated as BGMRC 2031T (= NBRC 111907T = KCTC 52119T). In addition, a novel family, Bruguierivoracaceae fam. nov., is proposed to accommodate the genera Bruguierivorax, Biostraticola, and Sodalis. The online version of this article (10.1007/s00284-020-02311-w) contains supplementary material, which is available to authorized users.
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