From allergen to oral vaccine carrier: A new face of ragweed pollen.
From allergen to oral vaccine carrier: A new face of ragweed pollen.
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DOI：
10.1016/j.ijpharm.2018.05.003
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发表时间：
2018-07-10
影响因子：
5.8
通讯作者：
Gill HS
中科院分区：
文献类型：
--
作者：
Uddin MJ;Gill HS
口服递送疫苗是非常可取的，但它取得了有限的成功。以前，我们开发了无过敏原的花粉粒作为口服疫苗接种的新方法。我们表明，石松孢子棒可以用于口服疫苗。然而，尚不清楚其他物种的花粉是否可以类似地用作口服疫苗载体。因此，在这项研究中，我们评估了普通豚草（RW）花粉（豚草）的口服疫苗接种潜力。以天然花粉为原料，经化学处理制备出无过敏原的RW花粉。用处理的RW配制的卵清蛋白（OVA）每周口服8次，产生强烈的全身（抗OVA IgG、IgG 1、IgG 2a和伊加）和粘膜（抗OVA伊加）免疫应答，在接种后至少持续3个月。在肺灌洗液、粪便、唾液和阴道分泌物中发现抗OVA的粘液伊加。此外，在最后一次免疫后三个半月，在骨髓中发现了主动分泌IgG和IgG1抗体的OVA特异性浆细胞。血清中未检测到针对RW特异性蛋白的IgE。总的来说，RW花粉证明了口服疫苗的潜力。
Oral delivery of vaccines is highly desirable, yet it has met with limited success. Previously we developed allergen-free pollen grains as a novel approach for oral vaccination. We showed that spores of Lycopodium clavatum can be used for oral vaccination. However, it is unknown if pollens of other species can be similarly used as an oral vaccine carrier. Therefore, in this study, we evaluated common ragweed (RW) pollen (Ambrosia elatior) for its oral vaccination potential. Allergen-free RW pollens were prepared from natural pollens through chemical treatment. Eight weekly oral doses of ovalbumin (OVA) formulated with treated RW generated strong systemic (anti-OVA IgG, IgG1, IgG2a, and IgA) and mucosal (anti-OVA IgA) immune responses that sustained for at least three months after vaccination. Mucosal IgA against OVA was found in the lung lavage, feces, saliva, and vaginal secretion. Moreover, three and half months after the last immunization OVA-specific plasma cells were found in the bone marrow that actively secreted IgG and IgG1 antibodies. No IgE against RW-specific proteins was detected in the serum. Overall, RW pollen demonstrated potential for oral vaccine delivery.
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