PPARγ/Pgc-1α-Fndc5 pathway up-regulation in gastrocnemius and heart muscle of exercised, branched chain amino acid diet fed mice.
PPARγ/Pgc-1α-Fndc5 pathway up-regulation in gastrocnemius and heart muscle of exercised, branched chain amino acid diet fed mice.
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DOI：
10.1186/s12986-018-0298-3
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发表时间：
2018
影响因子：
4.5
通讯作者：
Nasr-Esfahani MH
中科院分区：
文献类型：
--
作者：
Abedpoor N;Taghian F;Ghaedi K;Niktab I;Safaeinejad Z;Rabiee F;Tanhaei S;Nasr-Esfahani MH
先前的研究揭示了支链氨基酸（BCAA）分解代谢对脂肪酸氧化和代谢的诱导作用，特别是在肌肉细胞中。在本研究中，我们试图探讨将 BCAA 补充剂与有氧运动相结合是否可以改善雄性 C57BL/6 小鼠腓肠肌和心脏组织中 PPARγ、Pgc-1α 和 Fndc5 基因的表达。选择平均体重为18±2g的年轻雄性小鼠36只。将小鼠随机分为 6 组：20 mg/mL BCAA 消耗同时进行运动训练、60 mg/mL BCAA 消耗同时进行运动训练、经过运动训练但不消耗 BCAA 组、20 mg/mL BCAA 不进行运动训练、60 mg/mL BCAA 不进行运动训练以及未经训练且不消耗 BCAA 的小鼠。研究结果显示，20 mg/mL BCAA 与有氧运动的组合可显着增加骨骼肌中的 Fndc5、PPARγ、Pgc-1α 基因表达，但循环鸢尾素水平保持不变 (p<0.05)。有趣的是，给予 60 mg/mL BCAA 的小鼠进行运动后，血浆尿素和乳酸水平显着增加 (p<<0.05)。使用双向方差分析（ANOVA）来检查组与久坐组之间的显着差异。结果显示，20 mg/mL BCAA 对腓肠肌中 Fndc5、PPARγ、Pgc-1α 表达水平的诱导作用与心脏组织中的相应作用相似。值得注意的是，在运动期间补充 20 mg/mL BCAA 后，检测到较高的血清鸢尾素水平。补充支链氨基酸对骨骼肌和心脏代谢的概述 本文的在线版本 (10.1186/s12986-018-0298-3) 包含补充材料，可供授权用户使用。
Previous studies have revealed the inductive effect of branched-chain amino acids (BCAAs) catabolism on fatty acid oxidation and metabolism, especially in muscle cells. In the present investigation, we have attempted to address whether a combination of BCAAs supplement consumption with aerobic exercise could elaborate the expression of PPARγ, Pgc-1α and Fndc5 genes in gastrocnemius muscle and heart tissue of male C57BL/6 mice. Thirty-six young male mice with an average weight of 18 ± 2 g were selected. Mice were randomly assigned to 6 groups: 20 mg/mL of BCAAs consumption with simultaneous exercise-training, 60 mg/mL of BCAAs consumption with simultaneous exercise-training, exercise-trained with no BCAAs consumption group, 20 mg/mL BCAAs without exercise-training, 60 mg/mL BCAAs without exercise-training, and untrained mice without BCAAs consumption. The findings showed a combination of 20 mg/mL BCAAs with aerobic exercise significantly increased Fndc5, PPARγ, Pgc-1α gene expression in skeletal muscles although, circulating Irisin levels remained unchanged (p < 0.05). Interestingly, plasma urea and lactate levels were significantly increased in 60 mg/mL BCAAs administrated mice which performed exercised (p < 0.05). Two-way analysis of variance (ANOVA) was used to examine significant difference between groups and sedentary group. Results showed inductive effect of 20 mg/mL BCAAs on expression levels of Fndc5, PPARγ, Pgc-1α in gastrocnemius muscle similar with counterparts in heart tissue. Of note, higher serum irisin levels were detected after 20 mg/mL BCAAs supplementation coincided with the exercise. An Overview on supplemantaion of branched chain amoinoacids on metablism of skeletal muscle and heart The online version of this article (10.1186/s12986-018-0298-3) contains supplementary material, which is available to authorized users.
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影响因子：
3
作者：
Aydin, Suna;Kuloglu, Tuncay;Dabak, Ozlem
通讯作者：
Dabak, Ozlem
影响因子：
3.7
作者：
Hatazawa Y;Tadaishi M;Nagaike Y;Morita A;Ogawa Y;Ezaki O;Takai-Igarashi T;Kitaura Y;Shimomura Y;Kamei Y;Miura S
通讯作者：
Miura S
影响因子：
4.2
作者：
Harris, RA;Joshi, M;Obayashi, M
通讯作者：
Obayashi, M
影响因子：
4.6
作者：
Boivin GP;Platt KM;Corbett J;Reeves J;Hardy AL;Elenes EY;Charnigo RJ;Hunter SA;Pearson KJ
通讯作者：
Pearson KJ
影响因子：
5.9
作者：
Falavigna G;Alves de Araújo J Jr;Rogero MM;Pires IS;Pedrosa RG;Martins E Jr;Alves de Castro I;Tirapegui J
通讯作者：
Tirapegui J