Lipoprotein alterations in 10- and 20-week-old Zucker diabetic fatty rats: hyperinsulinemic versus insulinopenic hyperglycemia.
Lipoprotein alterations in 10- and 20-week-old Zucker diabetic fatty rats: hyperinsulinemic versus insulinopenic hyperglycemia.
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10 周和 20 周龄 Zucker 糖尿病脂肪大鼠的脂蛋白变化：高胰岛素血症与胰岛素缺乏性高血糖。
DOI：
10.1016/s0026-0495(98)90298-0
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Sparks,CE
中科院分区：
文献类型：
--
作者：
Sparks,JD;Phung,TL;Bolognino,M;Cianci,J;Khurana,R;Peterson,RG;Sowden,MP;Corsetti,JP;Sparks,CE
在10周龄和20周龄的雄性Zucker糖尿病肥胖（ZDF）大鼠中研究了脂蛋白和载脂蛋白参数，分别对应于高胰岛素血症和胰岛素缺乏的2型糖尿病。在两个年龄段，ZDF大鼠血清甘油三酯、游离脂肪酸和皮质酮升高，而20周的ZDF大鼠甲状腺激素降低。第10周时，高脂血症仅限于富含前β-环糊精（d < 1.006 g/mL）的脂蛋白升高。到20周时，与瘦大鼠相比，所有脂蛋白密度组分均增加，低密度脂蛋白（LDL）和高密度脂蛋白（HDL）胆固醇均大幅增加。在ZDF大鼠中，载脂蛋白B（apo B）从10周时的1.9倍对照组逐渐增加至20周时的3倍对照组。载脂蛋白B的增加伴随着载脂蛋白B（尤其是B100）从极低密度脂蛋白（VLDL）转变为对应于中密度脂蛋白加LDL的密度更高的脂蛋白（1.006 < d < 1.063 g/mL）。在Zucker和10周ZDF大鼠中，在存在高胰岛素血症的情况下，血清apo B的增加主要是VLDL中存在的apo B48。到20周时，当ZDF大鼠胰岛素减少时，B48：B100的质量比从2.7变为0.7。该变化与肝脏编辑的载脂蛋白B mRNA降低相关。载脂蛋白E增加瘦大鼠之间的10和20周龄。虽然ZDF大鼠的载脂蛋白E也增加，但20周时的增加低于瘦大鼠。ZDF大鼠VLDL中载脂蛋白E与B的摩尔比降低。在瘦大鼠中，HDL中存在超过50%的载脂蛋白E，而ZDF大鼠中HDL中存在不到20%的载脂蛋白E。VLDL载脂蛋白E在20周龄时转变为密度更高的组分，与载脂蛋白B相似。载脂蛋白C水平是瘦大鼠水平的两倍以上，并从HDL组分重新分布到含有载脂蛋白B的脂蛋白组分中。在ZDF大鼠中，载脂蛋白A-I和载脂蛋白A-IV水平在10至20周之间增加了一倍以上。ZDF大鼠模型可用于糖尿病从高胰岛素血症到胰岛素减少症进展过程中脂蛋白的比较研究。
Lipoprotein and apolipoprotein parameters were studied in the male Zucker diabetic fatty (ZDF) rat at 10 and 20 weeks of age, corresponding to hyperinsulinemic and insulinopenic type 2 diabetes mellitus, respectively. At both ages, ZDF rats had elevated serum triglycerides, free fatty acids, and corticosterone, whereas 20-week ZDF rats had reduced thyroid hormones. At 10 weeks, the hyperlipidemia was confined to elevations in pre-β triglyceride-rich (d < 1.006 g/mL) lipoproteins. By 20 weeks, all lipoprotein density fractions were increased compared with lean rats, with substantial increases in both low-density lipoprotein (LDL) and high-density lipoprotein (HDL) cholesterol. In ZDF rats, there was a progressive increase in apolipoprotein B (apo B) from 1.9 times control at 10 weeks to three times control at 20 weeks. The increase in apo B was accompanied by a shift of apo B, particularly B100, from very-low-density lipoprotein (VLDL) into denser lipoproteins corresponding to intermediate-density lipoproteins plus LDLs (1.006 < d < 1.063 g/mL). In Zucker and 10-week ZDF rats, in the presence of hyperinsulinemia, the increase in serum apo B was predominantly apo B48 present in VLDL. By 20 weeks, when ZDF rats are insulinopenic, the mass ratio of B48:B100 shifted from 2.7 to 0.7. The shift was associated with a decrease in hepatic-edited apo B mRNA. Apo E increased in lean rats between 10 and 20 weeks of age. Although apo E also increased in ZDF rats, the increase by 20 weeks was less than that of lean rats. The molar ratio of apo E to B in VLDL was decreased in ZDF rats. In lean rats, greater than 50% of apo E was present in HDL, in contrast to ZDF rats, where less than 20% of apo E was present in HDL. VLDL apo E shifted to denser fractions by 20 weeks of age, similar to apo B. The apo C level was more than double compared with the level in lean rats and was redistributed from the HDL fraction to lipoprotein fractions containing apo B. Both apo A-I and apo A-IV levels more than doubled between 10 and 20 weeks in ZDF rats. The ZDF rat model may be useful in comparative studies of lipoproteins during diabetic progression from hyperinsulinemia to insulinopenia.
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影响因子：
6.5
作者：
G. Schonfeld;C. Felski;M. A. Howald
通讯作者：
G. Schonfeld;C. Felski;M. A. Howald
转基因小鼠中人载脂蛋白 C-III 的过度表达会导致载脂蛋白 B48 残余物的积累，并被过量的载脂蛋白 E 纠正。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
deSilva,HV;Lauer,SJ;Wang,J;Simonet,WS;Weisgraber,KH;Mahley,RW;Taylor,JM
通讯作者：
Taylor,JM
胰岛素通过改变载脂蛋白 B mRNA 编辑来促进载脂蛋白 B-48 的生物合成和分泌。
DOI：
10.1073/pnas.91.12.5392
发表时间：
1994
影响因子：
11.1
作者：
Thorngate,FE;Raghow,R;Wilcox,HG;Werner,CS;Heimberg,M;Elam,MB
通讯作者：
Elam,MB
通过磷钨酸/MgCl2 沉淀来定量高密度脂蛋白胆固醇。
影响因子：
9.3
作者：
G. Assmann;H. Schriewer;Gerd Schmltz;Edgar
通讯作者：
Edgar
瘦和肥胖 Zucker 大鼠血清代谢物和激素的昼夜变化*
影响因子：
2.2
作者：
R. Martin;P. J. Wangsness;J. Gahagan
通讯作者：
J. Gahagan