Bacterial community composition shifts in the gut of Periplaneta americana fed on different lignocellulosic materials.
Bacterial community composition shifts in the gut of Periplaneta americana fed on different lignocellulosic materials.
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DOI：
10.1186/2193-1801-2-609
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Machado EA
中科院分区：
文献类型：
--
作者：
Bertino-Grimaldi D;Medeiros MN;Vieira RP;Cardoso AM;Turque AS;Silveira CB;Albano RM;Bressan-Nascimento S;Garcia ES;de Souza W;Martins OB;Machado EA
蟑螂是可以适应不同组成的饮食的昆虫，包括木质纤维素材料。这些化合物的消化是通过昆虫自身的酶和肠道共生体产生的酶来实现的。蟑螂肠道上不同的和模块化的细菌门的存在表明，这种昆虫可能是一个有趣的模型，以研究与消化不同的木质纤维素饮食的肠道细菌群落的组织。因此，暴露于这种饮食的昆虫的肠道相关细菌群落的多样性的变化可以提供关于如何改善半纤维素和纤维素分解系统的有用见解。在这项工作中，通过16S rRNA克隆文库的序列分析，我们比较了在野生型或保持两种不同的饮食：甘蔗渣和结晶纤维素收集的美洲大蠊肠道相关细菌的系统发育多样性和组成。与野生型蟑螂相比，这些高纤维饮食有利于某些细菌门的优势，如厚壁菌门。我们的数据显示，美洲大蠊肠道中的细菌多样性很高，群落主要由拟杆菌门、厚壁菌门、变形菌门和增效菌门组成。我们的数据表明，肠道细菌群落的组成和多样性可以通过饮食组成来调节。甘蔗渣和结晶纤维素喂养的动物中厚壁菌门的存在增加表明这些细菌强烈参与了蟑螂肠道中的木质纤维素消化。蟑螂是杂食性动物，可以在它们的饮食中加入不同成分的食物，包括木质纤维素材料。这些化合物的消化是通过昆虫自身的酶和肠道共生体产生的酶来实现的。然而，不同纤维含量的饮食对肠道细菌群落的影响以及这如何影响蟑螂的消化仍然不清楚。肠道上存在的一些细菌门表明，蟑螂可能是一个有趣的模型，以研究在消化不同的木质纤维素饲料的肠道细菌群落的组织。有关暴露于这种饮食的昆虫的肠道相关细菌群落多样性变化的知识可以为如何改善半纤维素和纤维素分解系统提供有趣的见解。我们比较了蟑螂P. americana的系统发育多样性和肠道相关细菌的组成，这些蟑螂是在野外捕获的，也是在两种不同的饮食中饲养的：甘蔗渣和结晶纤维素。为了这个目的，我们构建了细菌16S rRNA基因文库，这表明，饮食中富含纤维素和甘蔗渣有利于优势的一些细菌门，更显着厚壁菌门，相比野生蟑螂。稀疏分析，LIBSHUFF和UniFrac PCA比较表明，野生昆虫的基因文库是最多样化的，其次是甘蔗渣喂养，然后纤维素喂养的动物。还值得注意的是，纤维素和甘蔗渣基因文库彼此相似。我们的数据显示，美洲大蠊肠道中的细菌多样性很高，群落主要由拟杆菌门、厚壁菌门、变形菌门和增效菌门组成。肠道细菌群落的组成和多样性可以通过食物组成的类型来调节。甘蔗渣和结晶纤维素喂养的动物中厚壁菌门的存在增加表明这些细菌强烈参与了蟑螂肠道中的木质纤维素消化。
Cockroaches are insects that can accommodate diets of different composition, including lignocellulosic materials. Digestion of these compounds is achieved by the insect’s own enzymes and also by enzymes produced by gut symbionts. The presence of different and modular bacterial phyla on the cockroach gut tract suggests that this insect could be an interesting model to study the organization of gut bacterial communities associated with the digestion of different lignocellulosic diets. Thus, changes in the diversity of gut associated bacterial communities of insects exposed to such diets could give useful insights on how to improve hemicellulose and cellulose breakdown systems. In this work, through sequence analysis of 16S rRNA clone libraries, we compared the phylogenetic diversity and composition of gut associated bacteria in the cockroach Periplaneta americana collected in the wild-types or kept on two different diets: sugarcane bagasse and crystalline cellulose. These high fiber diets favor the predominance of some bacterial phyla, such as Firmicutes, when compared to wild-types cockroaches. Our data show a high bacterial diversity in P. americana gut, with communities composed mostly by the phyla Bacteroidetes, Firmicutes, Proteobacteria and Synergistetes. Our data show that the composition and diversity of gut bacterial communities could be modulated by diet composition. The increased presence of Firmicutes in sugarcane bagasse and crystalline cellulose-fed animals suggests that these bacteria are strongly involved in lignocellulose digestion in cockroach guts. Cockroaches are omnivorous animals that can incorporate in their diets food of different composition, including lignocellulosic materials. Digestion of these compounds is achieved by the insect’s own enzymes and also by enzymes produced by gut symbiont. However, the influence of diet with different fiber contents on gut bacterial communities and how this affects the digestion of cockroaches is still unclear. The presence of some bacterial phyla on gut tract suggests that cockroaches could be an interesting model to study the organization of gut bacterial communities during digestion of different lignocellulosic diets. Knowledge about the changes in diversity of gut associated bacterial communities of insects exposed to such diets could give interesting insights on how to improve hemicellulose and cellulose breakdown systems. We compared the phylogenetic diversity and composition of gut associated bacteria in the cockroach P. americana caught on the wild or kept on two different diets: sugarcane bagasse and crystalline cellulose. For this purpose we constructed bacterial 16S rRNA gene libraries which showed that a diet rich in cellulose and sugarcane bagasse favors the predominance of some bacterial phyla, more remarkably Firmicutes, when compared to wild cockroaches. Rarefaction analysis, LIBSHUFF and UniFrac PCA comparisons showed that gene libraries of wild insects were the most diverse, followed by sugarcane bagasse fed and then cellulose fed animals. It is also noteworthy that cellulose and sugarcane bagasse gene libraries resemble each other. Our data show a high bacterial diversity in P. americana gut, with communities composed mostly by the phyla Bacteroidetes, Firmicutes, Proteobacteria and Synergistetes. The composition and diversity of gut bacterial communities could be modulated by font of diet composition. The increased presence of Firmicutes in sugarcane bagasse and crystalline cellulose-fed animals suggests that these bacteria are strongly involved in lignocellulose digestion in cockroach guts.
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影响因子：
3.7
作者：
Gilbert JA;Field D;Swift P;Thomas S;Cummings D;Temperton B;Weynberg K;Huse S;Hughes M;Joint I;Somerfield PJ;Mühling M
通讯作者：
Mühling M
影响因子：
4.4
作者：
GIJZEN, HJ;BARUGAHARE, M
通讯作者：
BARUGAHARE, M
影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.
影响因子：
3.6
作者：
Grieco, Maria Angela B.;Cavalcante, Janaina J. V.;Martins, Orlando B.
通讯作者：
Martins, Orlando B.
影响因子：
4.4
作者：
Hongoh, Y;Deevong, P;Kudol, T
通讯作者：
Kudol, T