Activatable superparamagnetic iron oxide nanoparticles scavenge reactive oxygen species in macrophages and endothelial cells.
Activatable superparamagnetic iron oxide nanoparticles scavenge reactive oxygen species in macrophages and endothelial cells.
复制标题
巨噬细胞和内皮细胞中可活化的超磁铁氧化铁纳米颗粒清除活性氧。
作者：
活性氧 (ROS) 是炎症的关键标志物，不同水平的超氧化物表明炎症程度。炎症性疾病仍然是发达国家死亡的主要原因。此前，我们证明互聚物复合的超顺磁性氧化铁纳米粒子（IPC-SPIO）能够在富含超氧化物的环境中解络并激活 T2 磁共振（MR）对比。在这里，我们研究了 IPC-SPIO 清除免疫细胞和内皮细胞中 ROS 的能力，这应该激活超顺磁核心。在外源产生的超氧化物中，纳米颗粒清除 ROS 的能力与浓度有关，范围为可用 ROS 的 5% 至 50% 以上。与聚（乙二醇）包被的 SPIO（PEG-SPIO）相比，在 IPCSPIO 存在的情况下，观察到 ROS 显着降低。在体外细胞测定中，在巨噬细胞、单核细胞和人内皮细胞中观察到 ROS 减少。与单核细胞相比，巨噬细胞和内皮细胞的 ROS 减少量明显更高。在大多数研究中，ROS 清除在暴露于 IPC-SPIO 后 12 小时达到峰值，随着 IPC-SPIO 浓度的增加，一些细胞样品的清除时间延长。在测试浓度下，颗粒不具有细胞毒性，共焦成像显示颗粒在细胞内的定位。这些发现证明了 IPC-SPIO 作为可激活 MR 造影剂的潜力，能够在炎症诱导的细胞氧化还原条件下激活，作为炎症疾病严重程度或分期的报告者。复合的 IPC-SPIO 内化后清除细胞内 ROS。
Reactive oxygen species (ROS) are key markers of inflammation, with varying levels of superoxide indicating the degree of inflammation. Inflammatory diseases remain the leading cause of death in the developed world. Previously, we showed that interpolymer complexed superparamagnetic iron oxide nanoparticles (IPC-SPIOs) are capable of decomplexing and activating T2 magnetic resonance (MR) contrast in superoxide-rich environments. Here, we investigate the ability of IPC-SPIOs to scavenge ROS in immune and endothelial cells which should activate the superparamagnetic core. In exogenously generated superoxide, ROS scavenging by the nanoparticles was concentration dependent and ranged from 5% to over 50% of available ROS. A statistically significant reduction in ROS was observed in the presence of IPCSPIOs compared to poly(ethylene glycol)-coated SPIOs (PEG-SPIOs). During in vitro cellular assays, a reduction in ROS was observed in macrophages, monocytes, and human endothelial cells. Macrophages and endothelial cells experienced significantly higher ROS reduction compared to monocytes. ROS scavenging peaked 12 hours post-exposure to IPC-SPIOs in most studies, with some cell samples experiencing extended scavenging with increasing IPC-SPIO concentration. At the tested concentrations, particles were not cytotoxic, and confocal imaging showed localization of particles within cells. These findings demonstrate the potential of IPC-SPIOs as activatable MR contrast agents capable of activating under inflammation-induced cellular redox conditions as reporters of inflammatory disease severity or staging. Complexed IPC-SPIOs scavenge intracellular ROS after internalization.
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影响因子：
3.4
作者：
Cheng, H. A.;Drinnan, C. T.;Fisher, O. Z.
通讯作者：
Fisher, O. Z.
影响因子：
4.7
作者：
Luo, J;Borgens, R;Shi, R
通讯作者：
Shi, R
银纳米颗粒的静脉内给药可通过细胞内ROS相关的内皮间结的损失引起器官毒性。
影响因子：
10
作者：
Guo H;Zhang J;Boudreau M;Meng J;Yin JJ;Liu J;Xu H
通讯作者：
Xu H
影响因子：
4
作者：
Harijith A;Ebenezer DL;Natarajan V
通讯作者：
Natarajan V
DOI：
10.1134/s0006297916110134
发表时间：
2016-11
期刊：
Biochemistry. Biokhimiia
影响因子：
--
作者：
Fatkhullina AR;Peshkova IO;Koltsova EK
通讯作者：
Koltsova EK