Genomic insight into the scale specialization of the biological control agent Novius pumilus (Weise, 1892).
Genomic insight into the scale specialization of the biological control agent Novius pumilus (Weise, 1892).
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对生物防治剂小方瓢虫（Novius pumilus (Weise, 1892)）的鳞片特化的基因组学见解
DOI：
10.1186/s12864-022-08299-w
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发表时间：
2022-01-31
期刊：
影响因子：
4.4
通讯作者：
Pang H
中科院分区：
文献类型：
--
作者：
Tang XF;Huang YH;Li HS;Chen PT;Yang HY;Liang YS;Du XY;Liu ZH;Li EF;Yang YC;Pang H
新瓢虫属Novius Mulsant，1846（= Rodolia Mulsant，1850）（鞘翅目，瓢虫科）在棉垫蚧的生物防治中起着重要作用。自从最著名的物种，vedalia甲虫Novius cardinalis（Mulsant，1850）从澳大利亚引入加州，一个多世纪以来，Novius的一些物种已经开始表现出一些领域的适应性，以新的，但相关的猎物物种。尽管它们在经济上很重要，但人们对与它们的饮食习惯相关的潜在遗传适应性知之甚少。了解Novius的基因组序列是进一步了解其生物学和在害虫防治中的潜在应用的重要一步。我们报告了Novius pumilus（Weise，1892）的第一个高质量的基因组序列，Novius的代表专家。基因家族进化的计算分析（CAFE）分析表明，几个编码化学传感器、消化和免疫相关酶的正交群在N. pumilus与其他四种瓢虫的基因组相比。此外，有些正交组还受到显著的正选择压力（P < 0.05）。值得注意的是，转录组分析表明，许多基因显着扩大和积极选择的正交组，以及与解毒相关的基因的差异表达，当N。pumilus取食自然猎物Icerya与不取食组相比。我们推测这些基因在Novius物种的Icerya适应中至关重要。我们报告了迄今为止第一个Novius基因组。此外，我们还为N. pumilus。本研究的结果有助于理解化学感受、消化、解毒和免疫等基因的进化与捕食性昆虫适应猎物的关系。这将为今后Novius在生物防治中的研究和利用提供参考。此外，了解捕食适应的可能分子机制也为N. pumilus和其他Novius，这可能有利于害虫防治。在线版本包含补充材料，可通过10.1186/s12864-022-08299-w获得。
Members of the genus Novius Mulsant, 1846 (= Rodolia Mulsant, 1850) (Coleoptera, Coccinellidae), play important roles in the biological control of cotton cushion scale pests, especially those belonging to Icerya. Since the best-known species, the vedalia beetle Novius cardinalis (Mulsant, 1850) was introduced into California from Australia, more than a century of successful use in classical biological control, some species of Novius have begun to exhibit some field adaptations to novel but related prey species. Despite their economic importance, relatively little is known about the underlying genetic adaptations associated with their feeding habits. Knowledge of the genome sequence of Novius is a major step towards further understanding its biology and potential applications in pest control. We report the first high-quality genome sequence for Novius pumilus (Weise, 1892), a representative specialist of Novius. Computational Analysis of gene Family Evolution (CAFE) analysis showed that several orthogroups encoding chemosensors, digestive, and immunity-related enzymes were significantly expanded (P < 0.05) in N. pumilus compared to the published genomes of other four ladybirds. Furthermore, some of these orthogroups were under significant positive selection pressure (P < 0.05). Notably, transcriptome profiling demonstrated that many genes among the significantly expanded and positively selected orthogroups, as well as genes related to detoxification were differentially expressed, when N. pumilus feeding on the nature prey Icerya compared with the no feeding set. We speculate that these genes are vital in the Icerya adaptation of Novius species. We report the first Novius genome thus far. In addition, we provide comprehensive transcriptomic resources for N. pumilus. The results from this study may be helpful for understanding the association of the evolution of genes related to chemosensing, digestion, detoxification and immunity with the prey adaptation of insect predators. This will provide a reference for future research and utilization of Novius in biological control programs. Moreover, understanding the possible molecular mechanisms of prey adaptation also inform mass rearing of N. pumilus and other Novius, which may benefit pest control. The online version contains supplementary material available at 10.1186/s12864-022-08299-w.
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