In vivo imaging of microscopic structures in the rat retina.
In vivo imaging of microscopic structures in the rat retina.
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DOI：
10.1167/iovs.09-3675
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发表时间：
2009-12
影响因子：
4.4
通讯作者：
Porter J
中科院分区：
文献类型：
--
作者：
Geng Y;Greenberg KP;Wolfe R;Gray DC;Hunter JJ;Dubra A;Flannery JG;Williams DR;Porter J
在啮齿动物体内分解单个视网膜细胞的能力在啮齿动物视觉系统和视网膜疾病模型中有应用。我们描述了荧光自适应光学扫描激光检眼镜（fAOSLO）的性能，它提供了大鼠视网膜的细胞和亚细胞成像。通过玻璃体内注射腺相关病毒载体，在正常大鼠视网膜神经节细胞中表达绿色荧光蛋白（eGFP）。使用fAOSLO同时获得反射和荧光视网膜图像。fAOSLO分辨率是通过比较体内图像与随后使用共聚焦显微镜从同一只眼睛获得的视网膜切片成像来表征的。视网膜毛细血管和egfp标记的神经节细胞体、树突和轴突在体内用自适应光学（AO）清晰地分辨出来。AO校正使总均方根波前误差平均从0.30 μm减小到0.05 μm （1.7 mm瞳孔）。平均体内线扩散函数（LSF）的半最大值全宽度（FWHM）为~ 1.84 μm，比衍射受限LSF的半最大值全宽度（FWHM）大约82%。在完美的像差补偿下，由于大数值孔径（~ 0.43），大鼠眼的体内分辨率可以比人眼高2倍。虽然fAOSLO矫正了相当一部分大鼠眼睛的像差，但对视网膜图像质量的直接测量显示，有些模糊超出了衍射的预期。尽管如此，亚细胞特征可以被解析，为利用AO在体内高分辨率地研究啮齿动物的眼睛提供了希望。
The ability to resolve single retinal cells in rodents in vivo has applications in rodent models of the visual system and retinal disease. We have characterized the performance of a fluorescence adaptive optics scanning laser ophthalmoscope (fAOSLO) that provides cellular and subcellular imaging of rat retina in vivo. Green fluorescent protein (eGFP) was expressed in retinal ganglion cells of normal Sprague Dawley rats via intravitreal injections of adeno-associated viral vectors. Simultaneous reflectance and fluorescence retinal images were acquired using the fAOSLO. fAOSLO resolution was characterized by comparing in vivo images with subsequent imaging of retinal sections from the same eyes using confocal microscopy. Retinal capillaries and eGFP-labeled ganglion cell bodies, dendrites, and axons were clearly resolved in vivo with adaptive optics (AO). AO correction reduced the total root mean square wavefront error, on average, from 0.30 μm to 0.05 μm (1.7-mm pupil). The full width at half maximum (FWHM) of the average in vivo line-spread function (LSF) was ∼1.84 μm, approximately 82% greater than the FWHM of the diffraction-limited LSF. With perfect aberration compensation, the in vivo resolution in the rat eye could be ∼2× greater than that in the human eye due to its large numerical aperture (∼0.43). While the fAOSLO corrects a substantial fraction of the rat eye's aberrations, direct measurements of retinal image quality reveal some blur beyond that expected from diffraction. Nonetheless, subcellular features can be resolved, offering promise for using AO to investigate the rodent eye in vivo with high resolution.
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影响因子：
4.1
作者：
Morgan, JE;Uchida, H;Caprioli, J
通讯作者：
Caprioli, J
影响因子：
1.8
作者：
CHAUDHURI, A;HALLETT, PE;PARKER, JA
通讯作者：
PARKER, JA
影响因子：
4.4
作者：
Greenberg, Kenneth P.;Geller, Scott F.;Flannery, John G.
通讯作者：
Flannery, John G.
影响因子：
1.8
作者：
de la Cera, Elena Garcia;Rodriguez, Guadalupe;Marcos, Susana
通讯作者：
Marcos, Susana
视网膜神经节细胞变性是拓扑的，但在DBA/2J小鼠中不是特异性细胞类型。
影响因子：
7.8
作者：
Jakobs, Tatjana C;Libby, Richard T;Ben, Yixin;John, Simon W M;Masland, Richard H
通讯作者：
Masland, Richard H