Near-Infrared Spectroscopy, a Rapid Method for Predicting the Age of Male and Female Wild-Type and Wolbachia Infected Aedes aegypti.
Near-Infrared Spectroscopy, a Rapid Method for Predicting the Age of Male and Female Wild-Type and Wolbachia Infected Aedes aegypti.
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DOI：
10.1371/journal.pntd.0005040
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发表时间：
2016-10
影响因子：
3.8
通讯作者：
Devine GJ
中科院分区：
文献类型：
--
作者：
Sikulu-Lord MT;Milali MP;Henry M;Wirtz RA;Hugo LE;Dowell FE;Devine GJ
估计蚊子种群的年龄分布对于评估其传播疾病的能力和评估现有病媒控制计划的有效性至关重要。这项研究报告了近红外光谱 (NIRS) 技术快速预测登革热和寨卡病毒主要媒介埃及伊蚊年龄的能力。使用这种方法对野生型雄性和雌性以及感染 wMel 和 wMelPop 菌株 Wolbachia pipientis 的雄性和雌性的年龄进行了表征。使用偏最小二乘 (PLS) 回归从其头部和胸部收集的光谱进行校准。研究发现蚊子的真实年龄和预测年龄之间存在高度显着的相关性。所有年龄组野生型雌性和雄性的决定系数分别为 R2 = 0.84 和 0.78。 wMel 和 wMelPop 感染女性年龄的决定系数分别为 0.71 和 0.80（两种情况下 P < 0.001）。野生型雌性伊蚊的年龄。埃及伊蚊可被识别为 < 或 ≥ 8 天，准确率为 91% (N = 501)，而雌性伊蚊则被识别为 8 天以下。感染 wMel 和 wMelPop 的埃及伊蚊被区分为两个年龄组，准确率分别为 83% (N = 284) 和 78% (N = 229)。我们的结果还表明 NIRS 可以区分年轻和年老的雄性野生型、wMel 和 wMelPop 感染的伊蚊。埃及伊蚊的准确率分别为 87% (N = 253)、83% (N = 277) 和 78% (N = 234)。我们已经证明了 NIRS 作为雌性和雄性野生型以及感染沃尔巴克氏体的伊蚊年龄预测因子的潜力。实验室条件下的埃及蚊子。经过现场验证，该工具有可能为登革热和寨卡病媒控制计划的监测提供廉价且快速的替代方案。埃及伊蚊是登革热、基孔肯雅热和寨卡病毒的主要传播媒介。这些病毒需要在蚊子体内发育一段时间才能传播给人类。根据环境因素，登革热和寨卡病毒在蚊子体内复制平均需要 8-10 天，而基孔肯雅病毒需要 2-7 天才能复制。因此，蚊子的年龄是疾病传播的关键决定因素。利用内共生细菌 Wolbachia pipientis 的蚊子控制策略已被证明可以有效阻止伊蚊的登革热传播。埃及蚊子。为了有效阻断病毒，感染沃尔巴克氏体 wMel 菌株的蚊子必须存活足够长的时间，以覆盖受感染蚊子的外在潜伏期 (EIP)，而感染 wMelPop 的蚊子预计寿命会缩短，以限制病毒复制的时间。因此，这些策略需要使用年龄分级技术进行常规监测。在这项研究中，我们研究了快速且具有成本效益的近红外光谱 (NIRS) 技术作为野生型和沃尔巴克氏体感染伊蚊的替代年龄分级工具的适用性。埃及蚊子。我们表明 NIRS 可以快速预测男性和女性 Ae 的平均年龄。埃及伊蚊的真实年龄相差±3天，可以确定哪些蚊子年龄足够大，具有潜在的传染性，准确度高达 91%。
Estimating the age distribution of mosquito populations is crucial for assessing their capacity to transmit disease and for evaluating the efficacy of available vector control programs. This study reports on the capacity of the near-infrared spectroscopy (NIRS) technique to rapidly predict the ages of the principal dengue and Zika vector, Aedes aegypti. The age of wild-type males and females, and males and females infected with wMel and wMelPop strains of Wolbachia pipientis were characterized using this method. Calibrations were developed using spectra collected from their heads and thoraces using partial least squares (PLS) regression. A highly significant correlation was found between the true and predicted ages of mosquitoes. The coefficients of determination for wild-type females and males across all age groups were R2 = 0.84 and 0.78, respectively. The coefficients of determination for the age of wMel and wMelPop infected females were 0.71 and 0.80, respectively (P< 0.001 in both instances). The age of wild-type female Ae. aegypti could be identified as < or ≥ 8 days old with an accuracy of 91% (N = 501), whereas female Ae. aegypti infected with wMel and wMelPop were differentiated into the two age groups with an accuracy of 83% (N = 284) and 78% (N = 229), respectively. Our results also indicate NIRS can distinguish between young and old male wild-type, wMel and wMelPop infected Ae. aegypti with accuracies of 87% (N = 253), 83% (N = 277) and 78% (N = 234), respectively. We have demonstrated the potential of NIRS as a predictor of the age of female and male wild-type and Wolbachia infected Ae. aegypti mosquitoes under laboratory conditions. After field validation, the tool has the potential to offer a cheap and rapid alternative for surveillance of dengue and Zika vector control programs. Aedes aegypti is the principal vector for dengue, chikungunya and Zika viruses. These viruses require a period of development inside the mosquito before they can be transmitted to humans. Depending on environmental factors, dengue and Zika viruses take an average of 8–10 days to replicate inside the mosquito whereas chikungunya virus takes 2–7 days to replicate. The age of mosquitoes is therefore a critical determinant of disease transmission. A mosquito control strategy utilizing an endosymbiotic bacterium Wolbachia pipientis, has been proven effective at blocking dengue transmission in Ae. aegypti mosquitoes. For effective virus blocking, mosquitoes infected with the wMel strain of Wolbachia must survive long enough to cover the extrinsic incubation period (EIP) of infected mosquitoes while those infected with wMelPop are expected to have a reduced lifespan to limit the period available for virus replication. These strategies therefore require routine monitoring using age grading techniques. In this study, we investigated the applicability of a rapid and cost effective near-infrared spectroscopy (NIRS) technique as an alternative age grading tool for wild-type and Wolbachia infected Ae. aegypti mosquitoes. We show that NIRS can rapidly predict on average, the age of male and female Ae. aegypti to ±3 days of their true age and can determine which mosquitoes are old enough to be potentially infectious with an accuracy of up to 91%.
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影响因子：
3.2
作者：
Mayagaya, Valeriana Simon;Ntamatungiro, Alex John;Maia, Marta Ferreira
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Maia, Marta Ferreira
影响因子：
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O'Neill, Scott L.
DOI：
10.4269/ajtmh.2006.74.462
发表时间：
2006-03-01
影响因子：
3.3
作者：
Hugo, LE;Kay, BH;Ryan, PA
通讯作者：
Ryan, PA