EGb761 improves histological and functional recovery in rats with acute spinal cord contusion injury
EGb761 improves histological and functional recovery in rats with acute spinal cord contusion injury
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EGb761改善急性脊髓挫伤大鼠的组织学和功能恢复
DOI：
10.1038/sc.2015.156
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发表时间：
2015-10
期刊：
影响因子：
2.2
通讯作者：
Luo, Z-J
中科院分区：
文献类型：
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作者：
Yang, M.;Ye, Z-X;Liang, W.;Luo, Z-J
研究设计：这是一项实验性研究。目的：本研究的目的是评估银杏叶提取物761（EGb 761）对损伤部位的组织学特征和对标准化脊髓损伤（SCI）大鼠的功能表现的神经保护作用。设置：本研究在陕西西安进行。方法：30只雌性Sprague-Dawley大鼠随机分为3组：假手术组、生理盐水对照组和EGb 761治疗组。计算Basso，Beattie，Bresnahan运动评分（BBB评分），并进行足迹分析以评价各组大鼠的功能表现。结果：与生理盐水对照组相比，EGb 761治疗组在伤后14 d，BBB评分均显著降低（P <0.05），而在伤后1、3、7 d，EGb 761治疗组的BBB评分均显著降低（P< 0.05）。EGb 761治疗组在14 DPI时也表现出步幅增加、步幅宽度减少和脚趾拖曳减少（P< 0.05）。HE染色结果显示，EGb 761治疗组与生理盐水对照组相比，损伤部位坏死减少（P< 0.05）。TUNEL和caspase-3染色分析表明，细胞凋亡在1-14 DPI时增加，在损伤后24小时在灰质中达到峰值，在白色物质中在7 DPI时达到峰值。在7 DPI，凋亡细胞的数量显着减少EGb 761治疗group.Conclusion：EGb 761管理在急性期脊髓损伤后显着减少继发性损伤引起的组织坏死和细胞凋亡，改善功能的表现。
Study design:This is an experimental study.Objectives:The objective of this study was to evaluate the neuroprotective effects of Ginkgo biloba extract 761 (EGb761) on histological features of injured sites and on functional performance of rats subjected to standardized spinal cord injury (SCI).Setting:This study was conducted in Xian, Shaanxi, China.Methods:Thirty female Sprague–Dawley rats were randomly divided into three groups: sham-operated, saline-treated control and EGb761-treated. The Basso, Beattie, Bresnahan Locomotor Rating Score (BBB score) was calculated and footprint analysis was performed to evaluate the functional performance of the rats in each group. Hematoxylin and eosin (HE) staining and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and caspase-3 staining were performed to evaluate the necrosis area and apoptotic cells at the injured site in each group.Results:At 14, but not 1, 3 and 7, days post injury (DPI), rats in the EGb761-treated group exhibited significantly better BBB scores compared with the saline-treated control group (P< 0.05). The EGb761-treated group also showed increased stride length, decreased stride width and reduced toe dragging at 14 DPI (P< 0.05). Analysis of HE staining revealed that the EGb761-treated group had reduced necrosis at the injury site compared with the saline-treated control group (P< 0.05). Analysis of TUNEL and caspase-3 staining demonstrated that cell apoptosis was increased at 1–14 DPI, peaking at 24-h post injury in the gray matter, and 7 DPI in the white matter. At 7 DPI, the quantity of apoptotic cells was significantly decreased in the EGb761-treated group.Conclusion:EGb761 administration during the acute phase after SCI significantly reduced secondary injury-induced tissue necrosis and cell apoptosis and improved functional performance in rats.
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影响因子：
--
作者：
J. Haleng;J. Pincemail;J. Defraigne;C. Charlier;J. Chapelle
通讯作者：
J. Haleng;J. Pincemail;J. Defraigne;C. Charlier;J. Chapelle
影响因子：
6.1
作者：
Nada, Shadia E.;Shah, Zahoor A.
通讯作者：
Shah, Zahoor A.
影响因子：
5.3
作者：
Beattie, MS;Bresnahan, JC;Young, W
通讯作者：
Young, W
影响因子：
2.5
作者：
Lingzhi Sun;Weiduan Zhuang;Xiangqing Xu;Jun Yang;Jing Teng;Fengxia Zhang
通讯作者：
Lingzhi Sun;Weiduan Zhuang;Xiangqing Xu;Jun Yang;Jing Teng;Fengxia Zhang
影响因子：
2.2
作者：
M. Yan;M. Yang;W. Shao;X-g Mao;B. Yuan;Y-f Chen;Z-x Ye;W. Liang;Z-j Luo
通讯作者：
M. Yan;M. Yang;W. Shao;X-g Mao;B. Yuan;Y-f Chen;Z-x Ye;W. Liang;Z-j Luo