Measurement of cortisol and cortisone in children's hair using ultra performance liquid chromatography and tandem mass spectrometry
Measurement of cortisol and cortisone in children's hair using ultra performance liquid chromatography and tandem mass spectrometry
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使用超高效液相色谱和串联质谱法测定儿童头发中的皮质醇和可的松
DOI：
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
J. Raul
中科院分区：
文献类型：
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作者：
B. Vanaelst;Noellie Rivet;I. Huybrechts;B. Ludes;S. Henauw;J. Raul
目的：建立一种标准化和有效的方法来测量头发中的皮质醇和皮质醇，可能会对儿童应激研究有所裨益，因为与其他常用的应激研究基质相比，这种生物基质是非侵入性的，容易获得，而且更实用。方法：建立了同时检测儿童头发中皮质醇和皮质醇的超高效液相色谱-串联质谱法(UPLC-MS/MS)。用UPLC-MS/MS方法对223名小学女生的6 cm头发样本进行了适用性检验，并计算了皮质醇/皮质醇的比值。结果：皮质醇和可的松的最低检出限和最低检出限分别为2pg mg−1和5pg mg−1。皮质醇和可的松在5-1000pg mg−-1(R2>；0.99)范围内线性关系良好，萃取回收率分别为>；84%和>；87%。方法间精密度的相对标准偏差百分比分别为13.4%(皮质醇)和14.1%(可的松)，批间精密度为18.8%。LOQ的日内和日间精确度的%BISAS值为18.9%，其他浓度的%BISA值为12.3%。皮质醇(N=39)、可的松(N=168)和皮质醇/可的松比值(N=31)的激素浓度中位数(PG mg−1)分别为8.80(5~1330.48)、8.6 2(5~69.6)和0.80(0.11~54.4)。结论：建立了一种同时测定6 cm头发中皮质醇和皮质醇的UPLC-MS/MS方法，具有较好的精密度和准确度。我们的观察表明，对于未来的研究，建议不要分析长达6厘米的完整毛发片段，因为片段间的丢失，而是限制在更近端的片段，这些片段可能更准确地代表HPA的活性。
Objectives: Children's stress research may benefit from the establishment of a standardized and validated methodology to measure cortisol and cortisone in hair, as this biomatrix is non-invasive, easily obtainable and more practicable compared to other commonly used matrices in stress research. Methods: A new analytical method using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) is developed and validated to simultaneously detect cortisol and cortisone in children's hair. Applicability of the UPLC-MS/MS method is tested in 6 cm hair samples of 223 elementary school girls, and cortisol-to-cortisone ratios are calculated. Results: The LOD and LOQ were 2 pg mg−1 and 5 pg mg−1 respectively, for both cortisol and cortisone. Good linear responses in the ranges 5–1000 pg mg−1 (R2 > 0.99) and extraction recoveries of >84% and >87% were observed for cortisol and cortisone respectively. Relative standard deviation percentages were <13.4% (cortisol) and <14.1% (cortisone) for intra-assay precision, and <18.8% for inter-assay precision for both analytes at LOQ. %Bias was <18.9% for intra- and inter-day accuracy at LOQ, and <12.3% at the other concentrations. The median hormone concentrations (pg mg−1, range) were 8.80 (5–1330.48), 8.62 (5–69.6) and 0.80 (0.11–54.4) for cortisol (N = 39), cortisone (N = 168) and the cortisol-to-cortisone ratio (N = 31) respectively. Conclusions: We presented a UPLC-MS/MS method with good precision and accuracy to simultaneously measure cortisol and cortisone in hair samples 6 cm long. Our observations suggest that it is advisable for future research not to analyse full hair segments up to 6 cm long because of inter-segment loss, but to restrict to more proximal fragments which may more accurately represent HPA activity.
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影响因子：
2.7
作者：
Ashley, N. T.;Barboza, P. S.;Wasser, S. K.
通讯作者：
Wasser, S. K.
影响因子：
2.7
作者：
Davenport, Matthew D.;Tiefenbacher, Stefan;Meyer, Jerrold S.
通讯作者：
Meyer, Jerrold S.
DOI：
10.1016/j.cca.2010.10.019
发表时间：
2011-01-30
期刊：
Clinica chimica acta; international journal of clinical chemistry
影响因子：
--
作者：
Hamel AF;Meyer JS;Henchey E;Dettmer AM;Suomi SJ;Novak MA
通讯作者：
Novak MA
DOI：
10.3109/10253890.2012.654479
发表时间：
2012-11-01
影响因子：
2.3
作者：
Dettenborn, L.;Tietze, A.;Stalder, T.
通讯作者：
Stalder, T.
影响因子：
3.7
作者：
Stalder, Tobias;Steudte, Susann;Kirschbaum, Clemens
通讯作者：
Kirschbaum, Clemens