Immunogenicity of plant-produced African horse sickness virus-like particles: implications for a novel vaccine.
Immunogenicity of plant-produced African horse sickness virus-like particles: implications for a novel vaccine.
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植物生产的非洲马疾病病毒样颗粒的免疫原性：对新型疫苗的影响。
DOI：
10.1111/pbi.12783
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发表时间：
2018-03
影响因子：
13.8
通讯作者：
Rybicki EP
中科院分区：
文献类型：
--
作者：
Dennis SJ;Meyers AE;Guthrie AJ;Hitzeroth II;Rybicki EP
非洲马瘟（Africa horses sickness，AHS）是一种致命的病毒性疾病，由非洲马瘟病毒（AHSV）引起。接种疫苗仍然是对抗AHS的最有效武器，因为除了良好的畜牧业外，没有治疗该病的方法。然而，唯一可商购的疫苗是病毒的减毒活疫苗（LAV）。该疾病在其流行地区以外爆发的威胁以及LAV未被许可在世界其他地方使用的事实，促使人们尝试开发一种替代的更安全，但具有成本效益的重组疫苗。在这里，我们报告了使用基于豇豆花叶病毒的HyperTrans（CPMV-HT）和相关的pEAQ植物表达载体系统，通过根癌农杆菌介导的所有四种衣壳蛋白在本氏烟草中的瞬时表达，以植物为基础生产病毒样颗粒（VLP）AHSV血清型5候选疫苗。生产工艺快速简单，可扩展，经济可行，最重要的是，针对疫苗产生的豚鼠抗血清在基于细胞的测定中显示出中和活病毒。据我们所知，这是第一份关于植物中产生的AHSV VLP的报告，这对遏制和对抗这种致命疾病的传播具有重要意义。
African horse sickness (AHS) is a debilitating and often fatal viral disease affecting horses in much of Africa, caused by the dsRNA orbivirus African horse sickness virus (AHSV). Vaccination remains the single most effective weapon in combatting AHS, as there is no treatment for the disease apart from good animal husbandry. However, the only commercially available vaccine is a live‐attenuated version of the virus (LAV). The threat of outbreaks of the disease outside its endemic region and the fact that the LAV is not licensed for use elsewhere in the world, have spurred attempts to develop an alternative safer, yet cost‐effective recombinant vaccine. Here, we report the plant‐based production of a virus‐like particle (VLP) AHSV serotype five candidate vaccine by Agrobacterium tumefaciens‐mediated transient expression of all four capsid proteins in Nicotiana benthamiana using the cowpea mosaic virus‐based HyperTrans (CPMV‐HT) and associated pEAQ plant expression vector system. The production process is fast and simple, scalable, economically viable, and most importantly, guinea pig antiserum raised against the vaccine was shown to neutralize live virus in cell‐based assays. To our knowledge, this is the first report of AHSV VLPs produced in plants, which has important implications for the containment of, and fight against the spread of, this deadly disease.
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影响因子：
5.5
作者：
Alberca, Berta;Bachanek-Bankowska, Katarzyna;Cabana, Marta;Calvo-Pinilla, Eva;Viaplana, Elisenda;Frost, Lorraine;Gubbins, Simon;Urniza, Alicia;Mertens, Peter;Castillo-Olivares, Javier
通讯作者：
Castillo-Olivares, Javier
影响因子：
5.5
作者：
Guthrie, Alan J.;Quan, Melvyn;MacLachlan, N. James
通讯作者：
MacLachlan, N. James
影响因子：
3.8
作者：
CHUMA, T;LEBLOIS, H;ROY, P
通讯作者：
ROY, P
影响因子：
5
作者：
Bekker, Shani;Burger, Pieter;van Staden, Vida
通讯作者：
van Staden, Vida
影响因子：
1.8
作者：
El Garch, H.;Crafford, J. E.;Minke, J. M.
通讯作者：
Minke, J. M.