Arsenic toxicity in the human nerve cell line SK-N-SH in the presence of chromium and copper.
Arsenic toxicity in the human nerve cell line SK-N-SH in the presence of chromium and copper.
复制标题
在铬和铜的存在下，人神经细胞系SK-N-SH中的砷毒性。
DOI：
10.1016/j.chemosphere.2013.01.005
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发表时间：
2013-05
期刊：
影响因子：
8.8
通讯作者：
Cai Y
中科院分区：
文献类型：
--
作者：
Hu L;Greer JB;Solo-Gabriele H;Fieber LA;Cai Y
As、Cr 和 Cu 代表多种金属/类金属暴露的一种潜在组合，因为这三种元素同时从经铬化砷酸铜 (CCA) 处理的木材（一种用于建筑施工的常见产品）中浸出，其含量可能具有潜在危害。本研究调查了经 CCA 处理的木材与 Cr 和 Cu 一起存在时 As 的神经毒性。使用人神经母细胞瘤细胞系 SK-N-SH 的细胞毒性模型评估毒性。将细胞用CCA处理的木材浸出液或单独含有砷酸盐[As(V)]、二价铜[Cu(II)]、三价铬[Cr(III)]或三种元素的不同组合的溶液培养。暴露 96 小时后，使用与对照相比细胞复制的变化来评估毒性。在木材浸出液中存在的三种元素中，砷在观察到的毒性作用中发挥了主要作用，其通过多种途径发挥作用，包括氧化应激的产生。 DOM 影响测试细胞中金属/类金属的吸收，但这似乎并没有明显影响毒性。当浓度低于美国 EPA 饮用水中允许的最大污染物水平时，Cu(II) 会增强毒性，Cr(III) 会抑制毒性。因此，如果仅基于砷的结果来评估真实环境中的砷毒性是不够的。
As, Cr, and Cu represent one potential combination of multiple metals/metalloids exposures since these three elements are simultaneously leached from chromated copper arsenate (CCA)-treated wood, a common product used for building construction, at levels that can be potentially harmful. This study investigated the neurotoxicity of As associated with CCA-treated wood when accompanied by Cr and Cu. The toxicity was evaluated on basis of a cytotoxicity model using human neuroblastoma cell line SK-N-SH. The cells were cultured with CCA-treated wood leachates or with solutions containing arsenate [As(V)], divalent copper [Cu(II)], trivalent chromium [Cr(III)] alone or in different combinations of the three elements. The toxicity was evaluated using variations in cell replication compared to controls after 96 hrs exposure. Among the three elements present in wood leachates, As played the primary role in the observed toxic effects, which exerted through multiple pathways, including the generation of oxidative stress. DOM affected the absorption of metals/metalloids into the test cells, which however did not obviously appear to impact toxicity. As toxicity was enhanced by Cu(II) and inhibited by Cr(III) at concentrations below U.S. EPA’s allowable maximum contaminant levels in drinking waters. Thus assessing As toxicity in real environments is not sufficient if based solely on the result from As.
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影响因子：
8.9
作者：
Hasan, A. Rasem;Hu, Ligang;Solo-Gabriele, Helena M.;Fieber, Lynne;Cai, Yong;Townsend, Timothy G.
通讯作者：
Townsend, Timothy G.
影响因子：
8.3
作者：
Calderón, J;Navarro, ME;Díaz-Barriga, F
通讯作者：
Díaz-Barriga, F
DOI：
10.1016/0742-8413(89)90021-2
发表时间：
1989-01-01
影响因子：
3.9
作者：
MASON, RW;EDWARDS, IR
通讯作者：
EDWARDS, IR
影响因子：
13.6
作者：
Song, JK;Dubey, B;Solo-Gabriele, H
通讯作者：
Solo-Gabriele, H
DOI：
10.1097/01.jom.0000169089.54549.db
发表时间：
2005-08-01
影响因子：
3.2
作者：
Hafeman, DM;Ahsan, H;Graziano, JH
通讯作者：
Graziano, JH