Piriform spider silk sequences reveal unique repetitive elements.
Piriform spider silk sequences reveal unique repetitive elements.
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DOI：
10.1021/bm1007585
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发表时间：
2010-11-08
影响因子：
6.2
通讯作者：
Lewis, Randolph V.
中科院分区：
文献类型：
--
作者：
Perry, David J.;Bittencourt, Daniela;Siltberg-Liberles, Jessica;Rech, Elibio L.;Lewis, Randolph V.
织珠蜘蛛丝纤维是由非常大的、高度重复的蛋白质组装而成的。重复的片段又包含短的、简单的重复氨基酸基序，这些基序解释了组装的纤维的物理和机械性质。在六种圆织丝纤蛋白中，梨状丝形成了连接盘，它连接着蛛网的关节，并将拖丝附着在表面上，这一点以前还没有被描述过。梨形丝蛋白cDNA是从三种昆虫的噬菌体文库中分离得到的，A. trifasciata、三带拟步行虫N. clavipes和N.残忍的从这些基因推导的氨基酸序列揭示了两个新的重复基序：交替脯氨酸基序，每隔一个氨基酸是脯氨酸，和富含谷氨酰胺的基序6至8个氨基酸。与其他蜘蛛丝蛋白类似，重复片段很大（>200个氨基酸），并且在物种内高度同质化。这三个物种的基因之间也存在大量的序列相似性，重复基序特别保守。北方杂交分析表明，该信使RNA大于11 kb，仅在梨状腺中表达。系统发育分析的新蛋白质的C-末端区域与已公布的蜘蛛丝蛋白有力地表明，pirifom序列形成一个直系同源群。
Orb-weaving spider silk fibers are assembled from very large, highly repetitive proteins. The repeated segments contain, in turn, short, simple repetitive amino acid motifs that account for the physical and mechanical properties of the assembled fiber. Of the six orb-weaver silk fibroins, the piriform silk that makes the attachment discs, which lashes the joints of the web and attaches dragline silk to surfaces has not been previously characterized. Piriform silk protein cDNAs were isolated from phage libraries of three species, A. trifasciata, N. clavipes, and N. cruentata. The deduced amino acid sequences from these genes revealed two new repetitive motifs: an alternating proline motif where every other amino acid is proline, and a glutamine-rich motif of 6 to 8 amino acids. Similar to other spider silk proteins, the repeated segments are large (>200 amino acids) and highly homogenized within a species. There is also substantial sequence similarity across the genes from the three species with particular conservation of the repetitive motifs. Northern blot analysis revealed that the messenger RNA is larger than 11kb and is expressed exclusively in the piriform glands of the spider. Phylogenetic analysis of the C-terminal regions of the new proteins with published spidroins robustly shows that the pirifom sequences form an ortholog group.
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影响因子：
56.9
作者：
Guerette, PA;Ginzinger, DG;Gosline, JM
通讯作者：
Gosline, JM
影响因子：
4
作者：
Hayashi, CY;Lewis, RV
通讯作者：
Lewis, RV
DOI：
10.1073/pnas.86.1.152
发表时间：
1989-01-01
影响因子：
11.1
作者：
HOLLEY, LH;KARPLUS, M
通讯作者：
KARPLUS, M
DOI：
10.1016/j.cbpb.2007.03.013
发表时间：
2007-08-01
影响因子：
2.2
作者：
Bittencourt, D.;Souto, B. M.;Rech, E. L.
通讯作者：
Rech, E. L.
影响因子：
5.6
作者：
Hayashi, CY;Lewis, RV
通讯作者：
Lewis, RV