Loci and pathways associated with uterine capacity for pregnancy and fertility in beef cattle.
Loci and pathways associated with uterine capacity for pregnancy and fertility in beef cattle.
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DOI：
10.1371/journal.pone.0188997
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Neibergs HL
中科院分区：
文献类型：
--
作者：
Neupane M;Geary TW;Kiser JN;Burns GW;Hansen PJ;Spencer TE;Neibergs HL
不育和低生育力对牛的经济和繁殖性能产生负面影响。值得注意的是，牛在怀孕的第一个月内发生显著的妊娠丢失，但对影响牛妊娠成功和丢失的遗传基因座知之甚少。为了鉴定与早期妊娠丢失相关的数量性状基因座（QTL），根据妊娠第28天的妊娠结果将安格斯杂交小母牛分类为连续胚胎移植。进行了全基因组关联分析（GWAA），比较了30头100%成功建立妊娠的高生育力小母牛与55头成功率为25%或更低的低生育力小母牛。进行基因集富集分析SNP（GSEA-SNP）以鉴定影响妊娠丢失的基因集和前沿基因。GWAA鉴定了22个QTL（p < 1 x 10−5），GSEA-SNP鉴定了9个基因集（标准化富集得分> 3.0），其中有253个前沿基因。网络分析确定TNF（肿瘤坏死因子）、雌激素和TP 53（肿瘤蛋白53）是671个上游调节子中的最高调节子（p < 0.001），而SOX 2（SRY [性别决定区Y]-盒2）和OCT 4（八聚体结合转录因子4）复合物是773个与生育力相关的主调节子中的最高主调节子（p < 0.001）。在提高早期妊娠成功率的途径中鉴定QTL和基因为基因组选择以提高牛的生育力提供了关键信息。鉴定的基因和调节因子还提供了深入了解牛妊娠建立的复杂生物学机制。
Infertility and subfertility negatively impact the economics and reproductive performance of cattle. Of note, significant pregnancy loss occurs in cattle during the first month of pregnancy, yet little is known about the genetic loci influencing pregnancy success and loss in cattle. To identify quantitative trait loci (QTL) with large effects associated with early pregnancy loss, Angus crossbred heifers were classified based on day 28 pregnancy outcomes to serial embryo transfer. A genome wide association analysis (GWAA) was conducted comparing 30 high fertility heifers with 100% success in establishing pregnancy to 55 subfertile heifers with 25% or less success. A gene set enrichment analysis SNP (GSEA-SNP) was performed to identify gene sets and leading edge genes influencing pregnancy loss. The GWAA identified 22 QTL (p < 1 x 10−5), and GSEA-SNP identified 9 gene sets (normalized enrichment score > 3.0) with 253 leading edge genes. Network analysis identified TNF (tumor necrosis factor), estrogen, and TP53 (tumor protein 53) as the top of 671 upstream regulators (p < 0.001), whereas the SOX2 (SRY [sex determining region Y]-box 2) and OCT4 (octamer-binding transcription factor 4) complex was the top master regulator out of 773 master regulators associated with fertility (p < 0.001). Identification of QTL and genes in pathways that improve early pregnancy success provides critical information for genomic selection to increase fertility in cattle. The identified genes and regulators also provide insight into the complex biological mechanisms underlying pregnancy establishment in cattle.
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DOI：
10.1530/jrf.0.0590205
发表时间：
1980-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
BETTERIDGE, KJ;EAGLESOME, MD;MITCHELL, D
通讯作者：
MITCHELL, D
泌乳和妊娠对荷斯坦母牛子宫内膜基因表达的影响在发情周期或妊娠的第17天。
影响因子：
3.5
作者：
Cerri RL;Thompson IM;Kim IH;Ealy AD;Hansen PJ;Staples CR;Li JL;Santos JE;Thatcher WW
通讯作者：
Thatcher WW
影响因子：
2.9
作者：
Cochran SD;Cole JB;Null DJ;Hansen PJ
通讯作者：
Hansen PJ
DOI：
10.1530/jrf.0.0590463
发表时间：
1980-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
DISKIN, MG;SREENAN, JM
通讯作者：
SREENAN, JM
影响因子：
2.8
作者：
Berg, D. K.;van Leeuwen, J.;Pfeffer, P. L.
通讯作者：
Pfeffer, P. L.