Discovery of single nucleotide polymorphisms in candidate genes associated with fertility and production traits in Holstein cattle.
Discovery of single nucleotide polymorphisms in candidate genes associated with fertility and production traits in Holstein cattle.
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DOI：
10.1186/1471-2156-14-49
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发表时间：
2013-06-07
期刊：
影响因子：
2.9
通讯作者：
Hansen PJ
中科院分区：
文献类型：
--
作者：
Cochran SD;Cole JB;Null DJ;Hansen PJ
识别与生殖有关的特定基因的单核苷酸多态(SNPs)可能会提高这些低遗传力性状基因组估计的可靠性。使用Sequenom Massarray®系统对550头高(≥1.7；n = 288)或低(≤−2；n = 262)的荷斯坦公牛的精液进行了434个候选SNPs的基因分型。对三种类型的SNPs进行了评估：先前报道的与生殖特征相关或物理上接近生殖遗传标记的SNPs，众所周知参与生殖过程的基因中的SNPs，以及在与生殖功能相关的各种组织中不同生理条件表达的基因中的SNPs。对11个繁殖和生产性状进行了分析。共有40个SNP(P <； )与DPR相关。其中涉及内分泌系统、细胞信号、免疫功能和抑制细胞凋亡的基因。共有10个基因受雌二醇调控。此外，与母牛受胎率、母牛受胎率、生产年限、净值、产奶量、产奶量、产脂率、蛋白质产量、蛋白质含量和体细胞评分相关的SNPs分别为22个、33个、36个、34个、23个、19个。SNPs的等位基因替代效应与母牛受胎率、母牛受胎率、生产年限和净值的影响方向一致。与生产性状相关的几个SNP的等位基因替代效应与DPR相反。尽管如此，有29个与DPR相关的SNP与生产性状没有负相关。共检测到40个与DPR相关的基因的SNPs，以及与其他性状相关的SNPs。将这些SNPs纳入生殖和其他性状的基因组测试中或许是可行的。与DPR相关的基因可能对理解生殖生理学很重要。鉴于与DPR相关的大量SNP与生产性状没有负相关，应该有可能在不影响生产的情况下选择DPR。
Identification of single nucleotide polymorphisms (SNPs) for specific genes involved in reproduction might improve reliability of genomic estimates for these low-heritability traits. Semen from 550 Holstein bulls of high (≥ 1.7; n = 288) or low (≤ −2; n = 262) daughter pregnancy rate (DPR) was genotyped for 434 candidate SNPs using the Sequenom MassARRAY® system. Three types of SNPs were evaluated: SNPs previously reported to be associated with reproductive traits or physically close to genetic markers for reproduction, SNPs in genes that are well known to be involved in reproductive processes, and SNPs in genes that are differentially expressed between physiological conditions in a variety of tissues associated in reproductive function. Eleven reproduction and production traits were analyzed. A total of 40 SNPs were associated (P < 0.05) with DPR. Among these were genes involved in the endocrine system, cell signaling, immune function and inhibition of apoptosis. A total of 10 genes were regulated by estradiol. In addition, 22 SNPs were associated with heifer conception rate, 33 with cow conception rate, 36 with productive life, 34 with net merit, 23 with milk yield, 19 with fat yield, 13 with fat percent, 19 with protein yield, 22 with protein percent, and 13 with somatic cell score. The allele substitution effect for SNPs associated with heifer conception rate, cow conception rate, productive life and net merit were in the same direction as for DPR. Allele substitution effects for several SNPs associated with production traits were in the opposite direction as DPR. Nonetheless, there were 29 SNPs associated with DPR that were not negatively associated with production traits. SNPs in a total of 40 genes associated with DPR were identified as well as SNPs for other traits. It might be feasible to include these SNPs into genomic tests of reproduction and other traits. The genes associated with DPR are likely to be important for understanding the physiology of reproduction. Given the large number of SNPs associated with DPR that were not negatively associated with production traits, it should be possible to select for DPR without compromising production.
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影响因子：
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