Serum Free Amino Acid Profiling in Differential Diagnosis of Ovarian Tumors-A Comparative Study with Review of the Literature.
Serum Free Amino Acid Profiling in Differential Diagnosis of Ovarian Tumors-A Comparative Study with Review of the Literature.
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DOI：
10.3390/ijerph18042167
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发表时间：
2021-02-23
影响因子：
--
通讯作者：
Kokot ZJ
中科院分区：
文献类型：
--
作者：
Horala A;Plewa S;Derezinski P;Klupczynska A;Matysiak J;Nowak-Markwitz E;Kokot ZJ
正确的术前卵巢癌（OC）诊断仍然具有挑战性。研究血清游离氨基酸 (SFAA) 谱，以确定 OC 的潜在新型生物标志物，并评估其在卵巢肿瘤鉴别诊断中的表现。根据组织病理学结果将血清样本分为：上皮性 OC (n = 38)、交界性卵巢肿瘤 (n = 6) 和良性卵巢肿瘤 (BOT) (n = 62)。使用基于高效液相色谱电喷雾电离串联质谱 (HPLC-ESI-MS/MS) 的 aTRAQ 方法评估 SFAA 谱。 OC+borderline 和 BOT 之间 11 种氨基酸的水平存在显着差异。组氨酸的受试者工作特征曲线下面积（AUC of ROC）最高（0.787）。胱氨酸和组氨酸被确定为早期 OC/BOT 和 I 型 OC 的最佳单一标记。对于晚期 OC，7 个氨基酸在各组之间存在显着差异，瓜氨酸获得最佳 AUC，为 0.807。 II 型 OC 和 BOT 之间，有 8 个氨基酸存在显着差异，组氨酸和瓜氨酸的 AUC 最高，为 0.798（AUC 为 0.778）。组氨酸被确定为卵巢肿瘤鉴别诊断中潜在的新生物标志物。将组氨酸与 CA125 和 HE4 一起添加到多标记物组中可改善 OC 和 BOT 之间的鉴别诊断。
Proper preoperative ovarian cancer (OC) diagnosis remains challenging. Serum free amino acid (SFAA) profiles were investigated to identify potential novel biomarkers of OC and assess their performance in ovarian tumor differential diagnosis. Serum samples were divided based on the histopathological result: epithelial OC (n = 38), borderline ovarian tumors (n = 6), and benign ovarian tumors (BOTs) (n = 62). SFAA profiles were evaluated using aTRAQ methodology based on high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). Levels of eleven amino acids significantly differed between OC+borderline and BOTs. The highest area under the receiver operating characteristic curve (AUC of ROC) (0.787) was obtained for histidine. Cystine and histidine were identified as best single markers for early stage OC/BOT and type I OC. For advanced stage OC, seven amino acids differed significantly between the groups and citrulline obtained the best AUC of 0.807. Between type II OC and BOTs, eight amino acids differed significantly and the highest AUC of 0.798 was achieved by histidine and citrulline (AUC of 0.778). Histidine was identified as a potential new biomarker in differential diagnosis of ovarian tumors. Adding histidine to a multimarker panel together with CA125 and HE4 improved the differential diagnosis between OC and BOTs.
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影响因子：
3.3
作者：
Miyagi, Etsuko;Maruyama, Yasuyo;Hirahara, Fumiki
通讯作者：
Hirahara, Fumiki
影响因子：
3.7
作者：
Miyagi Y;Higashiyama M;Gochi A;Akaike M;Ishikawa T;Miura T;Saruki N;Bando E;Kimura H;Imamura F;Moriyama M;Ikeda I;Chiba A;Oshita F;Imaizumi A;Yamamoto H;Miyano H;Horimoto K;Tochikubo O;Mitsushima T;Yamakado M;Okamoto N
通讯作者：
Okamoto N
影响因子：
29
作者：
Pavlova NN;Thompson CB
通讯作者：
Thompson CB
DOI：
10.1097/pas.0b013e3181cf3d79
发表时间：
2010-03
期刊：
The American journal of surgical pathology
影响因子：
--
作者：
Kurman RJ;Shih IeM
通讯作者：
Shih IeM
影响因子：
--
作者：
Koshiyama M;Matsumura N;Konishi I
通讯作者：
Konishi I