Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese Rats.
Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese Rats.
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低剂量的三氯蔗糖改变高脂饮食诱导的肥胖大鼠的粪便微生物群
DOI：
10.3389/fnut.2021.787055
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发表时间：
2021
影响因子：
5
通讯作者：
Ma J
中科院分区：
文献类型：
--
作者：
Zhang M;Chen J;Yang M;Qian C;Liu Y;Qi Y;Feng R;Yang M;Liu W;Ma J
人工甜味剂(AS)被广泛用作糖的替代品，以减少卡路里的摄入。然而，据报道，高剂量的砷通过调节肠道微生物区系而诱导糖耐量异常。本研究的目的是探讨低剂量三氯蔗糖对肥胖患者粪便微生物区系的影响。高脂饲养8周后，雄性SD大鼠随机分为4组，每组6只，分别给予生理盐水2ml(CON)、0.54 mM三氯蔗糖(N054)、0.78 mM三氯蔗糖(N078)和324 mM蔗糖(S324)。4周后取粪便标本进行16S核糖体RNA基因测序分析。三氯蔗糖和蔗糖不改变粪便微生物区系的丰富度和多样性。0.54 mM(0.43 mg)和0.78 mM(0.62 mg)三氯蔗糖均有降低益生菌、乳杆菌科和阿克曼草科细菌的趋势。经0.54 mM三氯蔗糖处理后，乳酸菌科及其属细菌的相对丰度均增加。在功能预测中，0.54 mM三氯蔗糖提高了碳水化合物代谢谱，而0.78 mM三氯蔗糖提高了氨基酸代谢谱。较低剂量的三氯蔗糖可能会改变粪便微生物区系的组成。三氯蔗糖在不同剂量下的作用应在今后的研究中考虑。
Artificial sweeteners (AS) have been widely used as sugar substitutes to reduce calorie intake. However, it was reported that high doses of AS induced glucose intolerance via modulating gut microbiota. The objective of this study was to investigate the effects of lower doses of sucralose on fecal microbiota in obesity. Eight weeks after high-fat diet (HFD), the male Sprague Dawley rats were randomly divided into four groups (6 in each group) and administrated by a daily gavage of 2 ml normal saline (CON), 0.54 mM sucralose (N054), 0.78 mM sucralose (N078), and 324 mM sucrose (S324), respectively. After 4 weeks, fecal samples were obtained and analyzed by 16S ribosomal RNA gene sequencing. The richness and diversity of fecal microbiota were not changed by sucralose or sucrose. Both 0.54 mM (0.43 mg) and 0.78 mM (0.62 mg) sucralose tended to reduce the beneficial bacteria, Lactobacillaceae and Akkermansiaceae. The relative abundance of family Acidaminoccaceae and its genus Phascolarctobacteriam were increased after 0.54 mM sucralose. In functional prediction, 0.54 mM sucralose increased profiles of carbohydrate metabolism, whereas 0.78 mM sucralose enhanced those of amino acid metabolism. The lower doses of sucralose might alter the compositions of fecal microbiota. The effects of sucralose in different dosages should be considered in the future study.
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影响因子：
64.8
作者：
通讯作者：
--
影响因子：
3.7
作者：
Lecomte V;Kaakoush NO;Maloney CA;Raipuria M;Huinao KD;Mitchell HM;Morris MJ
通讯作者：
Morris MJ
DOI：
10.1136/bmj.h3576
发表时间：
2015-07-21
期刊：
BMJ (Clinical research ed.)
影响因子：
--
作者：
Imamura F;O'Connor L;Ye Z;Mursu J;Hayashino Y;Bhupathiraju SN;Forouhi NG
通讯作者：
Forouhi NG
DOI：
10.3390/molecules23020367
发表时间：
2018-02-09
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Chi L;Bian X;Gao B;Tu P;Lai Y;Ru H;Lu K
通讯作者：
Lu K
影响因子：
82.9
作者：
Depommier, Clara;Everard, Amandine;Cani, Patrice D.
通讯作者：
Cani, Patrice D.