Effects of the Artificial Sweetener Neotame on the Gut Microbiome and Fecal Metabolites in Mice.
Effects of the Artificial Sweetener Neotame on the Gut Microbiome and Fecal Metabolites in Mice.
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DOI：
10.3390/molecules23020367
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发表时间：
2018-02-09
期刊：
影响因子：
--
通讯作者：
Lu K
中科院分区：
文献类型：
--
作者：
Chi L;Bian X;Gao B;Tu P;Lai Y;Ru H;Lu K
虽然人工甜味剂在食品工业中得到了广泛的应用，但其对人体健康的影响仍存在争议。众所周知，肠道微生物区系在人体代谢中起着关键作用，最近的研究表明，一些人工甜味剂，如糖精，会扰乱肠道微生物群，进一步影响宿主健康，如引起糖耐量异常。纽甜是一种相对较新的低热量、高强度的人工甜味剂，于2002年获得FDA批准。然而，纽甜对肠道细菌的具体作用仍不清楚。在这项研究中，我们结合高通量测序和气相色谱-质谱仪(GC-MS)代谢组学来研究纽甜对CD-1小鼠肠道微生物群和粪便代谢物的影响。我们发现，四周的新糖消费降低了肠道微生物组的α多样性和β多样性。菌落总数显著减少，拟杆菌数显著增加。通过未观察到状态重建(PICRUST)分析进行的群落系统发育调查也表明，对照小鼠和新奥甜处理小鼠具有不同的代谢模式，一些关键基因如丁酸合成基因减少。此外，食用新糖还改变了粪便代谢物的组成。戏剧性的是，服用纽甜的小鼠粪便中多种脂肪酸、脂肪和胆固醇的浓度一直高于对照组。然而，其他代谢物，如苹果酸和甘油酸，则大大减少。综上所述，我们的研究首次探索了纽甜对小鼠肠道微生物区系的特异性影响，其结果可能有助于我们理解肠道微生物群与纽甜之间的相互作用以及这种相互作用如何影响宿主的正常代谢。
Although artificial sweeteners are widely used in food industry, their effects on human health remain a controversy. It is known that the gut microbiota plays a key role in human metabolism and recent studies indicated that some artificial sweeteners such as saccharin could perturb gut microbiome and further affect host health, such as inducing glucose intolerance. Neotame is a relatively new low-caloric and high-intensity artificial sweetener, approved by FDA in 2002. However, the specific effects of neotame on gut bacteria are still unknown. In this study, we combined high-throughput sequencing and gas chromatography–mass spectrometry (GC-MS) metabolomics to investigate the effects of neotame on the gut microbiome and fecal metabolite profiles of CD-1 mice. We found that a four-week neotame consumption reduced the alpha-diversity and altered the beta-diversity of the gut microbiome. Firmicutes was largely decreased while Bacteroidetes was significantly increased. The Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis also indicated that the control mice and neotame-treated mice have different metabolic patterns and some key genes such as butyrate synthetic genes were decreased. Moreover, neotame consumption also changed the fecal metabolite profiles. Dramatically, the concentrations of multiple fatty acids, lipids as well as cholesterol in the feces of neotame-treated mice were consistently higher than controls. Other metabolites, such as malic acid and glyceric acid, however, were largely decreased. In conclusion, our study first explored the specific effects of neotame on mouse gut microbiota and the results may improve our understanding of the interaction between gut microbiome and neotame and how this interaction could influence the normal metabolism of host bodies.
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影响因子：
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发表时间：
2010-08
期刊：
International journal of pediatric obesity : IJPO : an official journal of the International Association for the Study of Obesity
影响因子：
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