The polar lipids of Clostridium psychrophilum, an anaerobic psychrophile.
The polar lipids of Clostridium psychrophilum, an anaerobic psychrophile.
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DOI：
10.1016/j.bbalip.2013.02.004
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发表时间：
2013-06
期刊：
影响因子：
--
通讯作者：
Goldfine H
中科院分区：
文献类型：
--
作者：
Guan Z;Tian B;Perfumo A;Goldfine H
我们已经研究了嗜冷梭菌，最近的特点嗜冷梭菌分离南极微生物垫的极性脂质。从接近最佳生长温度（+5 °C）和-5 °C的细胞中提取脂质，并通过二维薄层色谱和液相色谱-质谱联用进行分析。该物种的主要磷脂是：心磷脂、磷脂酰乙醇胺和磷脂酰甘油。磷脂酰丝氨酸和溶血磷脂酰乙醇胺被发现作为次要成分。最丰富的糖脂是单糖基二酰甘油（MGDRG）和二糖基二酰甘油（DGDRG）。后者仅见于在-5 °C下生长的细胞中。在-5 °C下生长的细胞中发现了N-乙酰葡糖胺双甘甘油的乙醇胺磷酸衍生物，在+5 °C下生长的细胞中发现了MGDRG的乙醇胺磷酸衍生物。除PS和MGDRG主要以二酰基形式存在外，所有脂质均以全酰基和缩醛磷脂（烷-1 ′-烯酰基）形式存在。在两个生长温度的脂质变化的意义进行了讨论。
We have examined the polar lipids of Clostridium psychrophilum, a recently characterized psychrophilic Clostridium isolated from an Antarctic microbial mat. Lipids were extracted from cells grown near the optimal growth temperature (+5 °C) and at −5 °C, and analyzed by two-dimensional thin layer chromatography and liquid chromatography coupled with mass spectrometry. The major phospholipids of this species are: cardiolipin, phosphatidylethanolamine, and phosphatidylglycerol. Phosphatidylserine and lyso-phosphatidylethanolamine were found as minor components. The most abundant glycolipids are a monoglycosyldiradylglycerol (MGDRG) and a diglycosyldiradylglycerol (DGDRG). The latter was only seen in cells grown at −5 °C. An ethanolamine-phosphate derivative of N-acetylglucosaminyldiradylglycerol was seen in cells grown at −5 °C and an ethanolamine-phosphate derivative of MGDRG was found in cells grown at +5 °C. All lipids were present in both the all acyl and plasmalogen (alk-1′-enyl acyl) forms with the exception of PS and MGDRG, which were predominantly in the diacyl form. The significance of lipid changes at the two growth temperatures is discussed.
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