3D printed microfluidic device for online detection of neurochemical changes with high temporal resolution in human brain microdialysate.
3D printed microfluidic device for online detection of neurochemical changes with high temporal resolution in human brain microdialysate.
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DOI：
10.1039/c9lc00044e
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发表时间：
2019-06-07
期刊：
影响因子：
6.1
通讯作者：
中科院分区：
文献类型：
--
作者：
本文介绍了一种机械坚固的3D打印微流体装置的设计、优化和制造，用于以微升/分钟的流速对微透析液流中的生物标志物进行高时间分辨率在线分析。该设备由具有安全的低容量连接的微流体通道组成，可以轻松集成用于生物标志物（如谷氨酸盐，葡萄糖和乳酸盐）的电化学生物传感器。解释了微流体通道制造的优化过程，包括不同类型的3D打印机，并量化了传感器响应时间的改善。该设备的时间分辨率的特征在于记录短乳酸浓度脉冲。该器械用于记录同时发生的谷氨酸、葡萄糖和乳酸浓度变化，模拟对脑脊液透析液中扩散去极化事件的生理反应。作为一项概念验证研究，该设备随后在重症监护室中用于脑损伤患者的在线监测，展示了其临床监测能力。
This paper presents the design, optimisation and fabrication of a mechanically robust 3D printed microfluidic device for the high time resolution online analysis of biomarkers in a microdialysate stream at micro-litre per minute flow rates. The device consists of a microfluidic channel with secure low volume connections that easily integrates electrochemical biosensors for biomarkers such as glutamate, glucose and lactate. The optimisation process of the microfluidic channel fabrication, including for different types of 3D printer, is explained and the resulting improvement in sensor response time is quantified. The time resolution of the device is characterised by recording short lactate concentration pulses. The device is employed to record simultaneous glutamate, glucose and lactate concentration changes simulating the physiological response to spreading depolarisation events in cerebrospinal fluid dialysate. As a proof-of-concept study, the device is then used in the intensive care unit for online monitoring of a brain injury patient, demonstrating its capabilities for clinical monitoring.
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影响因子：
2.9
作者：
Nandi, Pradyot;Scott, David E.;Desai, Dhara;Lunte, Susan M.
通讯作者：
Lunte, Susan M.
影响因子：
2.2
作者：
Hamaoui, Karim;Gowers, Sally;Papalois, Vassilios
通讯作者：
Papalois, Vassilios
影响因子：
3
作者：
Moussawi K;Riegel A;Nair S;Kalivas PW
通讯作者：
Kalivas PW
影响因子：
6.2
作者：
Nandi P;Lunte SM
通讯作者：
Lunte SM
影响因子：
7.8
作者：
Kennedy, Robert T.
通讯作者：
Kennedy, Robert T.