Development and optimization of an integrated PDMS based-microdialysis microchip electrophoresis device with on-chip derivatization for continuous monitoring of primary amines.
Development and optimization of an integrated PDMS based-microdialysis microchip electrophoresis device with on-chip derivatization for continuous monitoring of primary amines.
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DOI：
10.1002/elps.201200454
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发表时间：
2013-03
期刊：
影响因子：
2.9
通讯作者：
Lunte, Susan M.
中科院分区：
文献类型：
--
作者：
Nandi, Pradyot;Scott, David E.;Desai, Dhara;Lunte, Susan M.
本文介绍了一种全PDMS在线微透析-微芯片电泳-芯片衍生化和电泳分离技术，用于含伯胺分析物的近实时监测。芯片的每个部分分别进行了优化，并确定了每个组件对器件的时间分辨率，滞后时间和分离效率的影响。天冬氨酸和谷氨酸被用作测试分析物。用萘-2，3-二甲醛/氰化物（NDA/CN−）完成衍生化，并使用15 cm蛇形通道进行分离。使用LIF检测来检测分析物。
An all-PDMS on-line microdialysis-microchip electrophoresis with on-chip derivatization and electrophoretic separation for near real-time monitoring of primary amine-containing analytes is described. Each part of the chip was optimized separately, and the effect of each of the components on temporal resolution, lag time, and separation efficiency of the device was determined. Aspartate and glutamate were employed as test analytes. Derivatization was accomplished with naphthalene-2,3,-dicarboxyaldehyde/cyanide (NDA/CN−), and the separation was performed using a 15-cm serpentine channel. The analytes were detected using LIF detection.
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影响因子：
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