Detection of extensive cross-neutralization between pandemic and seasonal A/H1N1 Influenza Viruses using a pseudotype neutralization assay.
Detection of extensive cross-neutralization between pandemic and seasonal A/H1N1 Influenza Viruses using a pseudotype neutralization assay.
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DOI：
10.1371/journal.pone.0011036
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发表时间：
2010-06-09
期刊：
影响因子：
3.7
通讯作者：
Clavel F
中科院分区：
文献类型：
--
作者：
Labrosse B;Tourdjman M;Porcher R;LeGoff J;de Lamballerie X;Simon F;Molina JM;Clavel F
季节性和大流行性甲型H1N1流感病毒之间的交叉免疫仍然不确定。特别是，以前感染过季节性甲型H1N1病毒或接种过季节性甲型H1N1病毒的疫苗，能在多大程度上引发针对大流行性甲型H1N1流感的保护性免疫，目前尚不清楚。使用基于HIV-1的假病毒中和试验测量针对季节性A/H1N1（A/布里斯班/59/2007）和针对大流行性A/H1N1（A/加州/04/2009）的中和滴度。使用这种高灵敏度的检测方法，我们发现大部分从未接触过甲型H1N1流感病毒的受试者表达高水平的甲型H1N1流感病毒中和滴度。在甲型H1N1流感大流行中和和标准季节性甲型H1N1流感毒株中和之间发现了显著的相关性。在2008-2009年接受季节性流感疫苗接种的受试者中，测得的甲型H1N1流感大流行中和滴度显著较高。在60岁以上的受试者中也测得了较高的大流行中和滴度。我们的研究结果表明，季节性和大流行性A/H1N1流感病毒之间的保护性交叉免疫的程度可能比以前估计的更重要。这种交叉免疫可以为最近流感大流行相对温和的特征提供一种可能的解释。
Cross-immunity between seasonal and pandemic A/H1N1 influenza viruses remains uncertain. In particular, the extent that previous infection or vaccination by seasonal A/H1N1 viruses can elicit protective immunity against pandemic A/H1N1 is unclear. Neutralizing titers against seasonal A/H1N1 (A/Brisbane/59/2007) and against pandemic A/H1N1 (A/California/04/2009) were measured using an HIV-1-based pseudovirus neutralization assay. Using this highly sensitive assay, we found that a large fraction of subjects who had never been exposed to pandemic A/H1N1 express high levels of pandemic A/H1N1 neutralizing titers. A significant correlation was seen between neutralization of pandemic A/H1N1 and neutralization of a standard seasonal A/H1N1 strain. Significantly higher pandemic A/H1N1 neutralizing titers were measured in subjects who had received vaccination against seasonal influenza in 2008–2009. Higher pandemic neutralizing titers were also measured in subjects over 60 years of age. Our findings reveal that the extent of protective cross-immunity between seasonal and pandemic A/H1N1 influenza viruses may be more important than previously estimated. This cross-immunity could provide a possible explanation of the relatively mild profile of the recent influenza pandemic.
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DOI：
10.1136/bmj.b3928
发表时间：
2009-10-06
期刊：
BMJ (Clinical research ed.)
影响因子：
--
作者：
Garcia-Garcia L;Valdespino-Gómez JL;Lazcano-Ponce E;Jimenez-Corona A;Higuera-Iglesias A;Cruz-Hervert P;Cano-Arellano B;Garcia-Anaya A;Ferreira-Guerrero E;Baez-Saldaña R;Ferreyra-Reyes L;Ponce-de-León-Rosales S;Alpuche-Aranda C;Rodriguez-López MH;Perez-Padilla R;Hernandez-Avila M
通讯作者：
Hernandez-Avila M
影响因子：
168.9
作者：
Plennevaux, Eric;Sheldon, Eric;Denis, Martine
通讯作者：
Denis, Martine
影响因子：
17.1
作者：
Del Giudice, Giuseppe;Stittelaar, Koert J.;Rappuoli, Rino
通讯作者：
Rappuoli, Rino
DOI：
10.1073/pnas.0911580106
发表时间：
2009-12-01
影响因子：
11.1
作者：
Greenbaum, Jason A.;Kotturi, Maya F.;Peters, Bjoern
通讯作者：
Peters, Bjoern
影响因子：
158.5
作者：
Hancock, Kathy;Veguilla, Vic;Katz, Jacqueline M.
通讯作者：
Katz, Jacqueline M.