Biofunctionalization of silicone polymers using poly(amidoamine) dendrimers and a mannose derivative for prolonged interference against pathogen colonization.
Biofunctionalization of silicone polymers using poly(amidoamine) dendrimers and a mannose derivative for prolonged interference against pathogen colonization.
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使用聚（氨基胺）树突聚合物和甘露糖衍生物对病原体定殖的长期干扰对有机硅聚合物的生物功能化。
DOI：
10.1016/j.biomaterials.2011.02.056
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发表时间：
2011-07
期刊：
影响因子：
14
通讯作者：
Cai, Chengzhi
中科院分区：
文献类型：
--
作者：
Lopez, Analette I.;Kumar, Amit;Planas, Megan R.;Li, Yan;Nguyen, Thuy V.;Cai, Chengzhi
Despite numerous preventive strategies on bacterial adhesion, pathogenic biofilm formation remained the major cause of medical device-related infections. Bacterial interference is a promising strategy that uses pre-established biofilms of benign bacteria to serve as live, protective coating against pathogen colonization. However, the application of this strategy to silicone urinary catheters was hampered by low adherence of benign bacteria onto silicone materials. In this work, we present a general method for biofunctionalization of silicone (PDMS) as one of the most widely used materials for biomedical devices. We used mild CO2 plasma to activate PDMS surface followed by simple attachment of generation 5 (G5) poly(amidoamine) (PAMAM) dendrimers to generate an amino-terminated surface that were maintained even after storage in PBS buffer for 36 days. We then covalently attach a carboxy-terminated mannose derivative to the modified PDMS to promote the adherence of benign Escherichia coli 83972 expressing mannose-binding type 1 fimbriae. We demonstrated that dense, stable biofilms of E. coli 83972 could be established within 48 h on the mannose-coated PDMS. Significantly, this benign biofilm reduced the adherence of the uropathogenic Enterococcus faecalis by 104-fold after 72 hours, while the benign bacteria on the unmodified substrate by only 5.5-fold.
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DOI：
10.1016/j.juro.2010.07.036
发表时间：
2010-12
期刊：
The Journal of urology
影响因子：
--
作者：
Desai DG;Liao KS;Cevallos ME;Trautner BW
通讯作者：
Trautner BW
影响因子：
6
作者：
Bodas, Dhananjay;Rauch, Jean-Yves;Khan-Malek, Chantal
通讯作者：
Khan-Malek, Chantal
影响因子：
4.5
作者：
Darouiche, Rabih O.;Riosa, Sarah;Hull, Richard A.
通讯作者：
Hull, Richard A.
影响因子：
2.1
作者：
Darouiche, RO;Donovan, WH;Hull, RA
通讯作者：
Hull, RA
影响因子：
2.8
作者：
Ferrieres, Lionel;Hancock, Viktoria;Klemm, Per
通讯作者：
Klemm, Per