A novel C-type lectin identified by EST analysis in tissue migratory larvae of Ascaris suum
A novel C-type lectin identified by EST analysis in tissue migratory larvae of Ascaris suum
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通过 EST 分析在猪蛔虫组织迁移幼虫中鉴定出一种新型 C 型凝集素
DOI：
10.1007/s00436-011-2677-9
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发表时间：
2012
影响因子：
2
通讯作者：
Maruyama H
中科院分区：
文献类型：
--
作者：
Yoshida A;Nagayasu E;Horii Y;Maruyama H
c型凝集素（ctl）是一组在Ca2+存在下与碳水化合物表位结合的蛋白质，已在广泛的物种中被描述。本研究从猪圆虫suumlung L3 （LL3）幼虫的cDNA文库中鉴定出一个推测的CTL编码cDNA序列，命名为asA。suumc型凝集素-1 （As-CTL-1）。As-CTL-1 cDNA的510个核苷酸开放阅读框编码了预测的169个氨基酸的蛋白，包括23个残基的推定信号肽和残基26至167的c型凝集素/ c型凝集素样结构域（CLECT）。as - ctl -1与弓形虫canc -type凝集素1和4 （Tc-CTL-1和4）最为相似，与命名型的ctl和哺乳动物的ctl高度同源，如人类C-type凝集素结构域家族4成员G （CLECG4）。此外，As-CTL-1在哺乳动物寄主体内的组织迁移期LL3和发育期L4幼虫中均有强烈表达。这些结果表明。由于As-CTL-1与宿主免疫细胞受体相似，因此幼虫可能利用As-CTL-1避开哺乳动物宿主的病原体识别机制。
C-type lectins (CTLs) are a group of proteins which bind to carbohydrate epitopes in the presence of Ca2+, which have been described in a wide range of species. In this study, a cDNA sequence coding a putative CTL has been identified from the cDNA library constructed from the pig round wormAscaris suumlung L3 (LL3) larvae, which was designated asA. suumC-type lectin-1 (As-CTL-1). The 510 nucleotide open reading frame of As-CTL-1 cDNA encoded the predicted 169 amino acid protein including a putative signal peptide of 23 residues and C-type lectin/C-type lectin-like domain (CLECT) at residue 26 to 167. As-CTL-1 was most similar toToxocara canisC-type lectin-1 and 4 (Tc-CTL-1 and 4), and highly homologous to namatode CTLs and mammalian CTLs as well, such as human C-type lectin domain family 4 member G (CLECG4). In addition, As-CTL-1 was strongly expressed in tissue migrating LL3 and the L4 larvae, which were developmental larvae stages within the mammalian host. These results suggest thatA. suumlarvae might utilize As-CTL-1 to avoid pathogen recognition mechanisms in mammalian hosts due to it is similarity to host immune cell receptors.
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DOI：
10.1016/0005-2728(93)90190-q
发表时间：
1993-02-08
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
TAKAMIYA, S;KITA, K;AOKI, T
通讯作者：
AOKI, T
动物凝集素对 Ca(2) 依赖性糖的识别。
影响因子：
3.9
作者：
Drickamer,K
通讯作者：
Drickamer,K
影响因子：
2.7
作者：
Islam, M. Khyrul;Miyoshi, Takeharu;Tsuji, Naotoshi
通讯作者：
Tsuji, Naotoshi
DOI：
10.1016/s0021-9258(17)40709-5
发表时间：
1994-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
S. Iobst;K. Drickamer
通讯作者：
S. Iobst;K. Drickamer
影响因子：
2.4
作者：
Daub, J;Loukas, A;Blaxter, M
通讯作者：
Blaxter, M