Electroacupuncture: a new approach to open the blood-brain barrier in rats recovering from middle cerebral artery occlusion.
Electroacupuncture: a new approach to open the blood-brain barrier in rats recovering from middle cerebral artery occlusion.
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电针：大脑中动脉闭塞恢复大鼠血脑屏障的新方法
DOI：
10.1136/acupmed-2017-011496
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发表时间：
2018-12
期刊：
影响因子：
--
通讯作者：
Di Z
中科院分区：
文献类型：
--
作者：
Zhang J;Lin X;Zhou H;Chen Y;Xiao S;Jiao J;Zhao Y;Di Z
目的：观察电针对正常大鼠和实验性缺血再灌注（I/R）大鼠恢复期血脑屏障（BBB）的开放作用，并探讨其开放作用是否具有时间依赖性。另一个目的是确定这种方法是否可以诱导神经生长因子（NGF）通过血脑屏障的渗透。首先建立大鼠大脑中动脉阻塞（MCAO）模型。选择不同的刺激时间，观察电针（100 Hz频率，2 mA强度）在GV 20和GV 26对MCAO恢复3周后大鼠BBB的影响。注射2%伊文思蓝（EB）生理盐水。使用湿/干称重法测量脑含水量。采用分光光度法和激光共聚焦显微镜检测EB的渗透程度。然后给大鼠注射神经生长因子，用酶联免疫吸附法测定脑组织中神经生长因子的浓度。电针8 min后血脑屏障通透性增加最明显（P<0.05），这可能有利于药物（如NGF）的脑靶向给药。此外，这种作用似乎不会引起健康或MCAO大鼠的脑水肿（P>0.05）。电针MCAO大鼠GV 20和GV 26一定时间可增加血脑屏障通透性。
To examine for an opening effect on the blood–brain barrier (BBB) in intact rats and rats with experimental ischaemia-reperfusion (I/R) during the recovery period after various electroacupuncture (EA) treatments with different time courses, and to determine whether there is a time-dependent effect. An additional objective was to determine whether this method could induce the penetration of nerve growth factor (NGF) through the BBB. A middle cerebral artery occlusion (MCAO) model was first established. We chose different stimulation time courses and observed the effects of EA treatment (100 Hz frequency; 2 mA intensity) at GV20 and GV26 on the BBB in rats recovering from MCAO 3 weeks after modelling. The rats were injected with 2% Evans blue (EB) saline. The brain water content was measured using a wet/dry weighing method. The degree of penetration of EB was detected using spectrophotometry and laser confocal microscopy. The rats were then injected with NGF, and the concentration of NGF in the brain tissues was measured using ELISA. The increase in the BBB permeability was most notable following the 8 min EA stimulation (P<0.05), which may be advantageous for the targeted delivery of drugs (such as NGF) into the brain. Additionally, this effect did not appear to cause brain oedema (P>0.05) in healthy or MCAO rats. EA treatment for a certain stimulation time at GV20 and GV26 in MCAO rats can increase BBB permeability.
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