The fecal viral flora of wild rodents.
The fecal viral flora of wild rodents.
复制标题
野生啮齿动物的粪便病毒植物。
DOI：
10.1371/journal.ppat.1002218
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发表时间：
2011-09
期刊：
影响因子：
6.7
通讯作者：
Delwart EL
中科院分区：
文献类型：
--
作者：
Phan TG;Kapusinszky B;Wang C;Rose RK;Lipton HL;Delwart EL
啮齿动物与人类的频繁互动使它们成为人畜共患感染的常见来源。为了获得野生啮齿动物肠道病毒多样性的初步无偏差测量，我们对在加利福尼亚州和弗吉尼亚州收集的 105 只野生啮齿动物（小鼠、田鼠和大鼠）的粪便中部分纯化、随机扩增的病毒 RNA 和 DNA 进行了测序。我们鉴定了与哺乳动物病毒科圆环病毒科、小核糖核酸病毒科、小核糖核酸病毒科、星状病毒科、细小病毒科、乳头瘤病毒科、腺病毒科和冠状病毒科相关的频率递减序列。 17 个小环状 DNA 基因组含有一或两个与代表几个潜在新病毒科的圆环病毒科有远亲关系的复制酶基因。在小核糖核酸病毒科中，对两个新的候选属以及人类病原体爱知病毒的近亲进行了鉴定。鉴定了第一个小鼠 sapelovirus 和 picobirnaviruses 的片段，并确定了第一个小鼠星状病毒基因组的特征。还对小鼠乳头瘤病毒基因组以及新型腺病毒和腺病毒相关病毒的片段进行了测序。啮齿类动物粪便病毒组的第二大部分与浓核病毒科、虹彩病毒科、多DNA病毒科、Dicistroviriade、溴病毒科和Virgaviridae科的昆虫病毒相关，其次是纳米病毒科、双生病毒科、藻类DNA病毒科、Secoviridae、Partitiviridae中的植物病毒相关序列。 Tymoviridae、Alphaflexiviridae 和 Tombusviridae 科主要以昆虫和植物啮齿类动物为食。因此，对完整和部分病毒基因组的系统发育分析揭示了许多以前未报道的病毒物种、属和科。一些啮齿动物和人类病毒之间的密切遗传相似性可能反映了过去的人畜共患疾病。这项研究增加了我们对野生啮齿动物病毒多样性的了解，并强调了哺乳动物中存在大量尚未表征的病毒。啮齿动物是多种人畜共患病病毒的天然宿主，可引起人类严重疾病。我们使用公正的宏基因组方法来表征啮齿动物粪便中的病毒多样性。除了饮食来源的昆虫和植物病毒之外，哺乳动物病毒序列也丰富且多样。最值得注意的是，对多个新的环状病毒 DNA 家族、两个新的小核糖核酸病毒科属以及第一个鼠星状病毒和小核糖核酸病毒进行了表征。小鼠科布病毒是人类病原体爱知病毒的近亲。这项研究显着增加了啮齿动物中已知的真核病毒遗传多样性，并提供了对其肠道病毒组的初步描述。
The frequent interactions of rodents with humans make them a common source of zoonotic infections. To obtain an initial unbiased measure of the viral diversity in the enteric tract of wild rodents we sequenced partially purified, randomly amplified viral RNA and DNA in the feces of 105 wild rodents (mouse, vole, and rat) collected in California and Virginia. We identified in decreasing frequency sequences related to the mammalian viruses families Circoviridae, Picobirnaviridae, Picornaviridae, Astroviridae, Parvoviridae, Papillomaviridae, Adenoviridae, and Coronaviridae. Seventeen small circular DNA genomes containing one or two replicase genes distantly related to the Circoviridae representing several potentially new viral families were characterized. In the Picornaviridae family two new candidate genera as well as a close genetic relative of the human pathogen Aichi virus were characterized. Fragments of the first mouse sapelovirus and picobirnaviruses were identified and the first murine astrovirus genome was characterized. A mouse papillomavirus genome and fragments of a novel adenovirus and adenovirus-associated virus were also sequenced. The next largest fraction of the rodent fecal virome was related to insect viruses of the Densoviridae, Iridoviridae, Polydnaviridae, Dicistroviriade, Bromoviridae, and Virgaviridae families followed by plant virus-related sequences in the Nanoviridae, Geminiviridae, Phycodnaviridae, Secoviridae, Partitiviridae, Tymoviridae, Alphaflexiviridae, and Tombusviridae families reflecting the largely insect and plant rodent diet. Phylogenetic analyses of full and partial viral genomes therefore revealed many previously unreported viral species, genera, and families. The close genetic similarities noted between some rodent and human viruses might reflect past zoonoses. This study increases our understanding of the viral diversity in wild rodents and highlights the large number of still uncharacterized viruses in mammals. Rodents are the natural reservoir of numerous zoonotic viruses causing serious diseases in humans. We used an unbiased metagenomic approach to characterize the viral diversity in rodent feces. In addition to diet-derived insect and plant viruses mammalian viral sequences were abundant and diverse. Most notably, multiple new circular viral DNA families, two new picornaviridae genera, and the first murine astrovirus and picobirnaviruses were characterized. A mouse kobuvirus was a close relative to the Aichi virus human pathogen. This study significantly increases the known genetic diversity of eukaryotic viruses in rodents and provides an initial description of their enteric viromes.
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DOI：
10.1099/vir.0.022764-0
发表时间：
2010-10
期刊：
The Journal of general virology
影响因子：
--
作者：
Chu DK;Chin AW;Smith GJ;Chan KH;Guan Y;Peiris JS;Poon LL
通讯作者：
Poon LL
影响因子：
5.4
作者：
Blinkova, Olga;Kapoor, Amit;Delwart, Eric L.
通讯作者：
Delwart, Eric L.
影响因子：
4.8
作者：
Day JM;Ballard LL;Duke MV;Scheffler BE;Zsak L
通讯作者：
Zsak L
影响因子：
3.2
作者：
Breitbart, M;Hewson, I;Rohwer, F
通讯作者：
Rohwer, F
DOI：
10.1073/pnas.202488399
发表时间：
2002-10-29
影响因子：
11.1
作者：
Breitbart, M;Salamon, P;Rohwer, F
通讯作者：
Rohwer, F