Molecular characteristics of global β-lactamase-producing Enterobacter cloacae by genomic analysis.
Molecular characteristics of global β-lactamase-producing Enterobacter cloacae by genomic analysis.
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通过基因组分析全球产β-内酰胺酶阴沟肠杆菌的分子特征
DOI：
10.1186/s12866-022-02667-y
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发表时间：
2022-10-21
期刊：
影响因子：
4.2
通讯作者：
Cao, Xiaoli
中科院分区：
文献类型：
--
作者：
Hu, Jincao;Li, Jia;Liu, Chang;Zhang, Yan;Xie, Hui;Li, Chuchu;Shen, Han;Cao, Xiaoli
分析全球产β-内酰胺酶阴沟肠杆菌的特征，包括β-内酰胺酶的分布、序列类型（ST）以及质粒复制子。阴沟肠杆菌的所有基因组均从 GenBank 下载。检查基因组质量后，通过基因组注释研究β-内酰胺酶编码基因的分布。通过多位点序列分型（MLST）分析这些菌株的 ST。通过将这些基因组提交给基因组流行病学中心，进一步探索了质粒复制子的分布。这些菌株的分离信息是通过Per程序从GenBank中提取的。 276株菌株中共有272株被发现携带β-内酰胺酶编码基因。其中，鉴定出 23 种 β-内酰胺酶，其中 blaCMH（n = 130，47.8%）和 blaACT（n = 126，46.3%）为优势品种，blaVIM 鉴定出 9 种碳青霉烯水解 β-内酰胺酶（CHβLs）基因型(n = 29, 10.7%) 和 blaKPC (n = 24, 8.9%）是最主要的。此外，还检测到 272 种携带 β-内酰胺酶的阴沟肠杆菌的 115 个不同的 ST，以及 106 种产生 CHβL 的阴沟肠杆菌的 48 个不同的 ST。 ST873 (n = 27, 9.9%) 是最常见的 ST。此外，还鉴定了25种不同的质粒复制子，其中最常见的是IncHI2（n = 65，23.9%）、IncHI2A（n = 64，23.5%）和IncFII（n = 62，22.8%）。值得注意的是，CHβLs 产生菌株中质粒复制子 IncHI2 和 IncHI2A 的分布显着高于非 CHβLs 产生菌株中的分布（p<0.05）。几乎所有的阴沟肠杆菌都含有β-内酰胺酶编码基因。全球阴沟肠杆菌中，blaCMH和blaACT是主要的blaAmpC基因。 BlaTEM 和 blaCTX-M 是主要的 ESBL。 BlaKPC、blaVIM 和 blaNDM 是主要的 CHβL。此外，还鉴定出了不同的 ST 和不同的复制子。在线版本包含可在 10.1186/s12866-022-02667-y 获取的补充材料。
To analyze the characteristics of global β-lactamase-producing Enterobacter cloacae including the distribution of β-lactamase, sequence types (STs) as well as plasmid replicons. All the genomes of the E. cloacae were downloaded from GenBank. The distribution of β-lactamase encoding genes were investigated by genome annotation after the genome quality was checked. The STs of these strains were analyzed by multi-locus sequence typing (MLST). The distribution of plasmid replicons was further explored by submitting these genomes to the genome epidemiology center. The isolation information of these strains was extracted by Per program from GenBank. A total of 272 out of 276 strains were found to carry β-lactamase encoding genes. Among them, 23 varieties of β-lactamase were identified, blaCMH (n = 130, 47.8%) and blaACT (n = 126, 46.3%) were the most predominant ones, 9 genotypes of carbapenem-hydrolyzing β-lactamase (CHβLs) were identified with blaVIM (n = 29, 10.7%) and blaKPC (n = 24, 8.9%) being the most dominant ones. In addition, 115 distinct STs for the 272 ß-lactamase-carrying E. cloacae and 48 different STs for 106 CHβLs-producing E. cloacae were detected. ST873 (n = 27, 9.9%) was the most common ST. Furthermore, 25 different plasmid replicons were identified, IncHI2 (n = 65, 23.9%), IncHI2A (n = 64, 23.5%) and IncFII (n = 62, 22.8%) were the most common ones. Notably, the distribution of plasmid replicons IncHI2 and IncHI2A among CHβLs-producing strains were significantly higher than theat among non-CHβLs-producing strains (p < 0.05). Almost all the E. cloacae contained β-lactamase encoding gene. Among the global E. cloacae, blaCMH and blaACT were main blaAmpC genes. BlaTEM and blaCTX-M were the predominant ESBLs. BlaKPC, blaVIM and blaNDM were the major CHβLs. Additionally, diversely distinct STs and different replicons were identified. The online version contains supplementary material available at 10.1186/s12866-022-02667-y.
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影响因子：
5.2
作者：
Liu W;Dong H;Yan T;Liu X;Cheng J;Liu C;Zhang S;Feng X;Liu L;Wang Z;Qin S
通讯作者：
Qin S
影响因子：
14.9
作者：
Kim T;Seo HD;Hennighausen L;Lee D;Kang K
通讯作者：
Kang K
影响因子：
3.7
作者：
Garza-González E;Bocanegra-Ibarias P;Bobadilla-Del-Valle M;Ponce-de-León-Garduño LA;Esteban-Kenel V;Silva-Sánchez J;Garza-Ramos U;Barrios-Camacho H;López-Jácome LE;Colin-Castro CA;Franco-Cendejas R;Flores-Treviño S;Morfín-Otero R;Rojas-Larios F;Mena-Ramírez JP;Fong-Camargo MG;Morales-De-la-Peña CT;García-Mendoza L;Choy-Chang EV;Aviles-Benitez LK;Feliciano-Guzmán JM;López-Gutiérrez E;Gil-Veloz M;Barajas-Magallón JM;Aguirre-Burciaga E;López-Moreno LI;Martínez-Villarreal RT;Canizales-Oviedo JL;Cetina-Umaña CM;Romero-Romero D;Bello-Pazos FD;Barlandas-Rendón NRE;Maldonado-Anicacio JY;Bolado-Martínez E;Galindo-Méndez M;Perez-Vicelis T;Alavez-Ramírez N;Méndez-Sotelo BJ;Cabriales-Zavala JF;Nava-Pacheco YC;Moreno-Méndez MI;García-Romo R;Silva-Gamiño AR;Avalos-Aguilera AM;Santiago-Calderón MA;López-García M;Velázquez-Acosta MDC;Cobos-Canul DI;Vázquez-Larios MDR;Ortiz-Porcayo AE;Guerrero-Núñez AE;Valero-Guzmán J;Rosales-García AA;Ostos-Cantú HL;Camacho-Ortiz A
通讯作者：
Camacho-Ortiz A
影响因子：
3
作者：
Kocsis, Bela;Kocsis, Erika;Mazzariol, Annarita
通讯作者：
Mazzariol, Annarita
影响因子：
3
作者：
Marchi, Emanuele;Jones, Mathew;Klenerman, Paul;Frater, John;Magiorkinis, Gkikas;Belshaw, Robert
通讯作者：
Belshaw, Robert