Ultrasensitive Qbeta phage analysis using fluorescence correlation spectroscopy on an optofluidic chip.
Ultrasensitive Qbeta phage analysis using fluorescence correlation spectroscopy on an optofluidic chip.
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DOI：
10.1016/j.bios.2009.04.005
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发表时间：
2009-07-15
影响因子：
12.6
通讯作者：
Schmidt H
中科院分区：
文献类型：
--
作者：
Rudenko MI;Kühn S;Lunt EJ;Deamer DW;Hawkins AR;Schmidt H
我们展示了使用集成的光流体生物传感器在单个病毒水平上检测和分析Qβ噬菌体。质量在原子图数量级的单个Qβ病毒在其天然液体环境中在硅芯片上被感测和分析，而不需要病毒固定。通过荧光相关光谱（FCS）从荧光信号中提取病毒的扩散系数，发现其为15.90±1.50 μm2/s，与先前公布的值非常一致。同时观察到噬菌体的聚集和解体。FCS测定的病毒流速在60-300 μm/s范围内。这项研究表明，一个廉价的和便携式的传感器能够区分不同大小的病毒相当大的潜力。
We demonstrate detection and analysis of the Qβ bacteriophage on the single virus level using an integrated optofluidic biosensor. Individual Qβ phages with masses on the order of attograms were sensed and analyzed on a silicon chip in their natural liquid environment without the need for virus immobilization. The diffusion coefficient of the viruses was extracted from the fluorescence signal by means of fluorescence correlation spectroscopy (FCS) and found to be 15.90±1.50 μm2/s in excellent agreement with previously published values. The aggregation and disintegration of the phage were also observed. Virus flow velocities determined by FCS were in the 60-300 μm/s range. This study suggests considerable potential for an inexpensive and portable sensor capable of discrimination between viruses of different sizes.
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