UVC disinfects SARS-CoV-2 by induction of viral genome damage without apparent effects on viral morphology and proteins.
UVC disinfects SARS-CoV-2 by induction of viral genome damage without apparent effects on viral morphology and proteins.
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UVC通过诱导病毒基因组损伤来消毒SARS-CoV-2，而对病毒形态和蛋白质没有明显影响。
DOI：
10.1038/s41598-021-93231-7
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发表时间：
2021-07-05
影响因子：
4.6
通讯作者：
Aida Y
中科院分区：
文献类型：
--
作者：
Lo CW;Matsuura R;Iimura K;Wada S;Shinjo A;Benno Y;Nakagawa M;Takei M;Aida Y
严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）已成为全球大流行威胁，并造成严重的健康和经济负担。被污染的环境，如个人物品和房间表面，被认为具有病毒传播的可能性。紫外线C （UVC）具有杀菌和去除环境污染的能力。UVC灭活了SARS-CoV-2；然而，其潜在机制尚不清楚。实验证实，在500 μW/cm2剂量下，253.7 nm的UVC可在30 s内完全灭活SARS-CoV-2，并可使病毒的传染性降低10 - 4.9倍。对病毒刺突蛋白和核衣壳蛋白的免疫印迹分析表明，UVC处理对病毒蛋白没有损害。透射电镜观察到，即使在紫外线照射后病毒完全失去传染性，病毒颗粒形态仍保持完整。相比之下，UVC照射诱导的基因组损伤使用新开发的长反转录定量聚合酶链反应（RT-qPCR）测定，而不是传统的RT-qPCR。6种覆盖病毒全长基因组的长RT-PCR分析清楚地表明，病毒传染性与UVC辐射诱导的基因组损伤呈负相关（R2范围为0.75 ~ 0.96）。总之，这些结果提供了UVC通过诱导病毒基因组损伤来灭活SARS-CoV-2的证据。
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been a pandemic threat worldwide and causes severe health and economic burdens. Contaminated environments, such as personal items and room surfaces, are considered to have virus transmission potential. Ultraviolet C (UVC) light has demonstrated germicidal ability and removes environmental contamination. UVC has inactivated SARS-CoV-2; however, the underlying mechanisms are not clear. It was confirmed here that UVC 253.7 nm, with a dose of 500 μW/cm2, completely inactivated SARS-CoV-2 in a time-dependent manner and reduced virus infectivity by 10–4.9-fold within 30 s. Immunoblotting analysis for viral spike and nucleocapsid proteins showed that UVC treatment did not damage viral proteins. The viral particle morphology remained intact even when the virus completely lost infectivity after UVC irradiation, as observed by transmission electronic microscopy. In contrast, UVC irradiation-induced genome damage was identified using the newly developed long reverse-transcription quantitative-polymerase chain reaction (RT-qPCR) assay, but not conventional RT-qPCR. The six developed long RT-PCR assays that covered the full-length viral genome clearly indicated a negative correlation between virus infectivity and UVC irradiation-induced genome damage (R2 ranging from 0.75 to 0.96). Altogether, these results provide evidence that UVC inactivates SARS-CoV-2 through the induction of viral genome damage.
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影响因子：
3.7
作者：
Narita K;Asano K;Morimoto Y;Igarashi T;Nakane A
通讯作者：
Nakane A
DOI：
10.3390/v13030408
发表时间：
2021-03-05
期刊：
Viruses
影响因子：
--
作者：
Gidari A;Sabbatini S;Bastianelli S;Pierucci S;Busti C;Bartolini D;Stabile AM;Monari C;Galli F;Rende M;Cruciani G;Francisci D
通讯作者：
Francisci D
影响因子：
13.2
作者：
Chan, Jasper Fuk-Woo;Kok, Kin-Hang;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung
影响因子：
4.4
作者：
Bosshard, Franziska;Armand, Florence;Kohn, Tamar
通讯作者：
Kohn, Tamar
DOI：
10.1016/j.dsx.2020.04.020
发表时间：
2020-07-01
影响因子：
10
作者：
Astuti, Indwiani;Ysrafil
通讯作者：
Ysrafil