Paraneoplastic antigen-like 5 gene (PNMA5) is preferentially expressed in the association areas in a primate specific manner.
Paraneoplastic antigen-like 5 gene (PNMA5) is preferentially expressed in the association areas in a primate specific manner.
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DOI：
10.1093/cercor/bhp062
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发表时间：
2009-12
期刊：
影响因子：
--
通讯作者：
Yamamori T
中科院分区：
文献类型：
--
作者：
Takaji M;Komatsu Y;Watakabe A;Hashikawa T;Yamamori T
为了在分子水平上了解灵长类动物新皮质区的结构和功能之间的关系，我们通过限制性标志cDNA扫描（RLCS）筛选了猕猴新皮质区差异表达的基因。在这里，我们报告的副肿瘤抗原样5基因（PNMA5）的丰富表达的关联领域，但不是在初级感觉区，与初级视觉皮层的表达水平最低。原位杂交显示PNMA5 mRNA的表达仅限于第二层的初级感觉区。沿着腹侧视通路，从初级视区到高级视区，在兴奋性神经元中的表达逐渐增加。这种差异表达模式与我们先前报道的另一个富含结合区的基因视黄醇结合蛋白（RBP）mRNA非常相似。用于比较PNMA基因家族中的其他基因PNMA1、PNMA2、PNMA3和MOAP 1（PNMA4）的额外表达分析表明，它们在区域和层间广泛表达，但没有PNMA5的分化模式。在小鼠脑中，PNMA1仅微弱表达，而PNMA5未检测到。序列分析表明PNMA5基因在哺乳动物中存在差异。这些发现表明，在灵长类进化过程中，PNMA5在新皮层的关联区域中获得了某种专门的作用。
To understand the relationship between the structure and function of primate neocortical areas at a molecular level, we have been screening for genes differentially expressed across macaque neocortical areas by restriction landmark cDNA scanning (RLCS). Here, we report enriched expression of the paraneoplastic antigen-like 5 gene (PNMA5) in association areas but not in primary sensory areas, with the lowest expression level in primary visual cortex. In situ hybridization in the primary sensory areas revealed PNMA5 mRNA expression restricted to layer II. Along the ventral visual pathway, the expression gradually increased in the excitatory neurons from the primary to higher visual areas. This differential expression pattern was very similar to that of retinol-binding protein (RBP) mRNA, another association-area-enriched gene that we reported previously. Additional expression analysis for comparison of other genes in the PNMA gene family, PNMA1, PNMA2, PNMA3, and MOAP1 (PNMA4), showed that they were widely expressed across areas and layers but without the differentiated pattern of PNMA5. In mouse brains, PNMA1 was only faintly expressed and PNMA5 was not detected. Sequence analysis showed divergence of PNMA5 sequences among mammals. These findings suggest that PNMA5 acquired a certain specialized role in the association areas of the neocortex during primate evolution.
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DOI：
10.1098/rstb.1999.0541
发表时间：
1999-12-29
期刊：
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
Crick, F
通讯作者：
Crick, F
DOI：
10.1016/0013-4694(69)90212-0
发表时间：
1969-01-01
期刊：
ELECTROENCEPHALOGRAPHY AND CLINICAL NEUROPHYSIOLOGY
影响因子：
--
作者：
BIGNALL, KE;IMBERT, M
通讯作者：
IMBERT, M
影响因子：
5.3
作者：
Elston, GN
通讯作者：
Elston, GN
影响因子：
6.1
作者：
Evans, SJ;Choudary, PV;Akil, H
通讯作者：
Akil, H
影响因子：
2.5
作者：
Freese, JL;Amaral, DG
通讯作者：
Amaral, DG