Enhancement of musculocutaneous nerve reinnervation after vascular endothelial growth factor (VEGF) gene therapy.
Enhancement of musculocutaneous nerve reinnervation after vascular endothelial growth factor (VEGF) gene therapy.
复制标题
DOI：
10.1186/1471-2202-13-57
复制
发表时间：
2012-06-06
期刊：
影响因子：
2.4
通讯作者：
Dubový P
中科院分区：
文献类型：
--
作者：
Haninec P;Kaiser R;Bobek V;Dubový P
血管内皮生长因子（Vascular endothelial growth factor， VEGF）不仅是一种有效的血管生成因子，而且还能促进雪旺细胞轴突生长和增殖。本研究的目的是定量评估在质粒phVEGF转染和端到端（ETE）或端到端（ETS）神经吻合后，使用运动和初级感觉神经元重建肌皮神经（MCN）残端。将大鼠MCN远端残端转染质粒phVEGF、质粒单独转染或载体转染，并在te或ETS神经缝合后进行2个月的再神经移植。用氟红宝石和氟祖母绿逆行标记MCN残端后，估计运动神经元和背根神经节神经元的数量。根据再生轴突的密度、直径和髓鞘厚度、梳毛试验和肱二头肌湿重指数评估MCN残肢的神经再生情况。在相同条件下，免疫组织化学检测显示，与仅转染质粒或载体处理的对照树桩相比，转染phVEGF质粒的MCN树桩的雪旺细胞中VEGF增加。转染质粒phVEGF的MCN残肢与对照组残肢相比，在te神经缝合后，运动神经元和感觉神经元的数量有所增加。然而，MCN质粒转染phVEGF后，髓鞘轴突的形态计量学质量、修饰试验和湿重指数均显著改善。MCN质粒phVEGF转染的残肢与对照残肢相比，ETS神经吻合导致运动和感觉神经元的显著升高，这些神经元重新支配MCN。特别值得注意的是，向MCN残端发出侧枝的神经元数量增加了。与ETE神经吻合类似，转染phVEGF后，有髓鞘轴突形态学质量、行为学测试和肱二头肌湿重指数均显著提高。我们的研究结果表明，质粒转染MCN残端可以诱导雪旺细胞中VEGF蛋白的增加，从而在te和ETS神经缝合后产生更高质量的轴突再神经。与对照大鼠相比，这也与更好的湿重肱二头肌指数和功能测试有关。
Vascular endothelial growth factor (VEGF) is not only a potent angiogenic factor but it also promotes axonal outgrowth and proliferation of Schwann cells. The aim of the present study was to quantitatively assess reinnervation of musculocutaneous nerve (MCN) stumps using motor and primary sensory neurons after plasmid phVEGF transfection and end-to-end (ETE) or end-to-side (ETS) neurorrhaphy. The distal stump of rat transected MCN, was transfected with plasmid phVEGF, plasmid alone or treated with vehiculum and reinnervated following ETE or ETS neurorrhaphy for 2 months. The number of motor and dorsal root ganglia neurons reinnervating the MCN stump was estimated following their retrograde labeling with Fluoro-Ruby and Fluoro-Emerald. Reinnervation of the MCN stumps was assessed based on density, diameter and myelin sheath thickness of regenerated axons, grooming test and the wet weight index of the biceps brachii muscles. Immunohistochemical detection under the same conditions revealed increased VEGF in the Schwann cells of the MCN stumps transfected with the plasmid phVEGF, as opposed to control stumps transfected with only the plasmid or treated with vehiculum. The MCN stumps transfected with the plasmid phVEGF were reinnervated by moderately higher numbers of motor and sensory neurons after ETE neurorrhaphy compared with control stumps. However, morphometric quality of myelinated axons, grooming test and the wet weight index were significantly better in the MCN plasmid phVEGF transfected stumps. The ETS neurorrhaphy of the MCN plasmid phVEGF transfected stumps in comparison with control stumps resulted in significant elevation of motor and sensory neurons that reinnervated the MCN. Especially noteworthy was the increased numbers of neurons that sent out collateral sprouts into the MCN stumps. Similarly to ETE neurorrhaphy, phVEGF transfection resulted in significantly higher morphometric quality of myelinated axons, behavioral test and the wet weight index of the biceps brachii muscles. Our results showed that plasmid phVEGF transfection of MCN stumps could induce an increase in VEGF protein in Schwann cells, which resulted in higher quality axon reinnervation after both ETE and ETS neurorrhaphy. This was also associated with a better wet weight biceps brachii muscle index and functional tests than in control rats.
登录
查看更多内容
影响因子：
2.4
作者：
Dubový P;Raška O;Klusáková I;Stejskal L;Celakovský P;Haninec P
通讯作者：
Haninec P
影响因子：
4.1
作者：
Haninec, Pavel;Samal, Filip;Dubovy, Petr
通讯作者：
Dubovy, Petr
DOI：
10.1016/j.bbrc.2003.08.134
发表时间：
2003-10-10
影响因子：
3.1
作者：
Fukino, K;Sata, M;Nagai, R
通讯作者：
Nagai, R
DOI：
10.1016/0266-7681(94)90069-8
发表时间：
1994-06-01
期刊：
JOURNAL OF HAND SURGERY-BRITISH AND EUROPEAN VOLUME
影响因子：
--
作者：
LUNDBORG, G;ZHAO, Q;KERNS, JM
通讯作者：
KERNS, JM
影响因子：
2.1
作者：
Matsumoto, M;Hirata, H;Uchida, A
通讯作者：
Uchida, A