Gallbladder microbiota composition is associated with pancreaticobiliary and gallbladder cancer prognosis.
Gallbladder microbiota composition is associated with pancreaticobiliary and gallbladder cancer prognosis.
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DOI：
10.1186/s12866-022-02557-3
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发表时间：
2022-05-27
期刊：
影响因子：
4.2
通讯作者：
中科院分区：
文献类型：
--
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在过去的十年中，肠道的微生物种群及其与特定疾病的关系已经得到了广泛的研究。然而，描述胆汁微生物群的报告很少。本研究旨在通过16 S rRNA基因扩增子测序研究胰胆管癌和良性疾病患者的微生物群组成，并评估其作为胆管癌，胰腺癌和胆囊癌生物标志物的潜在价值。我们招募了被诊断为癌症、囊性病变和胰胆管炎症的患者。研究队列包括244例患者。我们从手术切除的胆囊的胆汁中提取了微生物组来源的DNA。根据病变位置和癌症类型，微生物组组成在α和β多样性方面没有显著差异。我们发现，弯曲杆菌属，柠檬酸杆菌属，Leptotrichia，肠杆菌属，Hungatella，分枝杆菌属，叶杆菌属和鞘氨醇单胞菌属的相对丰度在无淋巴结转移和有淋巴结转移的患者之间存在显著差异。某些微生物的相对丰度与总体生存预后之间存在显着相关性。这些微生物与胆管癌的预后良好相关，而与胰腺癌的预后不良相关，反之亦然。我们的研究结果表明，胰胆管癌患者的微生物组组成发生了变化，这可能是区分恶性肿瘤的生物标志物。在线版本包含补充材料，可通过10.1186/s12866-022-02557-3获得。
The microbial population of the intestinal tract and its relationship to specific diseases has been extensively studied during the past decade. However, reports characterizing the bile microbiota are rare. This study aims to investigate the microbiota composition in patients with pancreaticobiliary cancers and benign diseases by 16S rRNA gene amplicon sequencing and to evaluate its potential value as a biomarker for the cancer of the bile duct, pancreas, and gallbladder. We enrolled patients who were diagnosed with cancer, cystic lesions, and inflammation of the pancreaticobiliary tract. The study cohort comprised 244 patients. We extracted microbiome-derived DNA from the bile juice in surgically resected gallbladders. The microbiome composition was not significantly different according to lesion position and cancer type in terms of alpha and beta diversity. We found a significant difference in the relative abundance of Campylobacter, Citrobacter, Leptotrichia, Enterobacter, Hungatella, Mycolicibacterium, Phyllobacterium and Sphingomonas between patients without and with lymph node metastasis. There was a significant association between the relative abundance of certain microbes and overall survival prognosis. These microbes showed association with good prognosis in cholangiocarcinoma, but with poor prognosis in pancreatic adenocarcinoma, and vice versa. Our findings suggest that pancreaticobiliary tract cancer patients have an altered microbiome composition, which might be a biomarker for distinguishing malignancy. The online version contains supplementary material available at 10.1186/s12866-022-02557-3.
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