Correlation Analysis between Gut Microbiota Alterations and the Cytokine Response in Patients with Coronavirus Disease during Hospitalization.
Correlation Analysis between Gut Microbiota Alterations and the Cytokine Response in Patients with Coronavirus Disease during Hospitalization.
复制标题
冠状病毒病患者住院期间肠道菌群变化与细胞因子反应的相关性分析。
DOI：
10.1128/spectrum.01689-21
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发表时间：
2022-04-27
影响因子：
3.7
通讯作者：
Yotsuyanagi, Hiroshi
中科院分区：
文献类型：
--
作者：
Mizutani, Taketoshi;Ishizaka, Aya;Koga, Michiko;Ikeuchi, Kazuhiko;Saito, Makoto;Adachi, Eisuke;Yamayoshi, Seiya;Iwatsuki-Horimoto, Kiyoko;Yasuhara, Atsuhiro;Kiyono, Hiroshi;Matano, Tetsuro;Suzuki, Yutaka;Tsutsumi, Takeya;Kawaoka, Yoshihiro;Yotsuyanagi, Hiroshi
肠道微生物区系在冠状病毒疾病2019年(新冠肺炎)中的作用正在阐明中。在这里，我们分析了住院新冠肺炎患者微生物区系组成的时间变化以及炎症生物标志物/细胞因子与微生物区系的相关性。我们从22名住院的新冠肺炎患者中获取了粪便样本、血液样本和患者记录，并对发病过程中的粪便样本进行了16SRRNA亚基因组分析，并与40名健康个体的粪便样本进行了比较。我们分析了肠道微生物区系的变化与血浆促炎细胞因子水平的相关性。入院后即刻，观察到新冠肺炎患者与健康人肠道微生物区系的差异，主要包括芽孢杆菌和棒状杆菌丰富，梭状芽胞杆菌丰富。在整个住院期间，细菌图谱继续发生变化，产生短链脂肪酸的细菌减少，兼性厌氧细菌Escherichia-Shigella增加。属于铜绿假单胞菌类别的埃格特氏菌持续增加。新冠肺炎中富集的放线菌与干扰素-γ水平呈负相关，与gp130/sIL-6Rb水平呈正相关。即使在发病21 天后，生物失调仍在继续。住院新冠肺炎患者的肠道倾向于需氧环境。由于肠道微生物区系的组成与促炎细胞因子的水平有关，这一发现强调了需要了解病理如何与在新冠肺炎患者中观察到的特定肠道微生物区系的时间变化有关。重要性越来越多的证据表明，胃肠道和呼吸道的共生微生物区系调节局部和全身炎症(肠肺轴)。新冠肺炎主要是一种呼吸系统疾病，但微生物区系变化在该病发病机制中的参与尚不清楚。住院期间新冠肺炎患者的肠道菌群组成随时间发生变化，新冠肺炎患者的肠道趋向于需氧环境。肠道微生物区系的这些变化可能会导致肠道通透性增加，称为肠漏，使细菌和毒素进入循环系统，进一步加剧全身炎症反应。由于肠道微生物区系的组成与促炎细胞因子的水平有关，这一发现突显了了解病理与肠道环境的关系的必要性，包括在新冠肺炎患者中观察到的特定肠道微生物区系的时间变化。
The role of the intestinal microbiota in coronavirus disease 2019 (COVID-19) is being elucidated. Here, we analyzed the temporal changes in microbiota composition and the correlation between inflammation biomarkers/cytokines and microbiota in hospitalized COVID-19 patients. We obtained stool specimens, blood samples, and patient records from 22 hospitalized COVID-19 patients and performed 16S rRNA metagenomic analysis of stool samples over the course of disease onset compared to 40 healthy individual stool samples. We analyzed the correlation between the changes in the gut microbiota and plasma proinflammatory cytokine levels. Immediately after admission, differences in the gut microbiota were observed between COVID-19 patients and healthy subjects, mainly including enrichment of the classes Bacilli and Coriobacteriia and decrease in abundance of the class Clostridia. The bacterial profile continued to change throughout the hospitalization, with a decrease in short-chain fatty acid-producing bacteria including Faecalibacterium and an increase in the facultatively anaerobic bacteria Escherichia-Shigella. A consistent increase in Eggerthella belonging to the class Coriobacteriia was observed. The abundance of the class Clostridia was inversely correlated with interferon-γ level and that of the phylum Actinobacteria, which was enriched in COVID-19, and was positively correlated with gp130/sIL-6Rb levels. Dysbiosis was continued even after 21 days from onset. The intestines tended to be an aerobic environment in hospitalized COVID-19 patients. Because the composition of the gut microbiota correlates with the levels of proinflammatory cytokines, this finding emphasizes the need to understand how pathology is related to the temporal changes in the specific gut microbiota observed in COVID-19 patients. IMPORTANCE There is growing evidence that the commensal microbiota of the gastrointestinal and respiratory tracts regulates local and systemic inflammation (gut-lung axis). COVID-19 is primarily a respiratory disease, but the involvement of microbiota changes in the pathogenesis of this disease remains unclear. The composition of the gut microbiota of patients with COVID-19 changed over time during hospitalization, and the intestines tended to be an aerobic environment in hospitalized COVID-19 patients. These changes in gut microbiota may induce increased intestinal permeability, called leaky gut, allowing bacteria and toxins to enter the circulatory system and further aggravate the systemic inflammatory response. Since gut microbiota composition correlates with levels of proinflammatory cytokines, this finding highlights the need to understand how pathology relates to the gut environment, including the temporal changes in specific gut microbiota observed in COVID-19 patients.
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影响因子：
14.9
作者：
Klindworth A;Pruesse E;Schweer T;Peplies J;Quast C;Horn M;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
6.4
作者：
Vital M;Karch A;Pieper DH
通讯作者：
Pieper DH
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
2.9
作者：
Wong, JMW;de Souza, R;Jenkins, DJA
通讯作者：
Jenkins, DJA
影响因子：
56.9
作者：
Lamers, Mart M.;Beumer, Joep;Clevers, Hans
通讯作者：
Clevers, Hans