The vascular endothelium of the adipose tissue gives rise to both white and brown fat cells.
The vascular endothelium of the adipose tissue gives rise to both white and brown fat cells.
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DOI：
10.1016/j.cmet.2012.01.008
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发表时间：
2012-02-08
期刊：
影响因子：
29
通讯作者：
Cinti S
中科院分区：
文献类型：
--
作者：
Tran KV;Gealekman O;Frontini A;Zingaretti MC;Morroni M;Giordano A;Smorlesi A;Perugini J;De Matteis R;Sbarbati A;Corvera S;Cinti S
脂肪组织扩张涉及现有脂肪细胞的扩大，从定向前脂肪细胞形成新细胞，以及组织血管网络的协调发展。在这里，我们发现，经典的白色和棕色脂肪库的小鼠内皮细胞（EC）与周细胞，这是多能性的，可以潜在地产生前脂肪细胞的超微结构特征。使用VE-钙粘蛋白启动子的谱系追踪实验揭示了报告基因在EC中的定位，以及在白色和棕色脂肪库的前脂肪细胞和脂肪细胞中的定位。此外，毛细血管芽从人脂肪组织，其中主要有EC的特点，被发现表达Zfp 423，最近确定的标志物前脂肪细胞的决定。为了响应PPARγ激活，发芽细胞的内皮特征逐渐丧失，细胞形成结构和生物化学定义的脂肪细胞。这些数据共同支持了鼠和人脂肪细胞的内皮起源，提示了脂肪组织扩张期间脂肪生成和血管生成如何协调的模型。
Adipose tissue expansion involves the enlargement of existing adipocytes, the formation of new cells from committed preadipocytes, and the coordinated development of the tissue vascular network. Here we find that murine endothelial cells (EC) of classic white and brown fat depots share ultrastructural characteristics with pericytes, which are pluripotent and can potentially give rise to preadipocytes. Lineage tracing experiments using the VE-cadherin promoter reveal localization of reporter genes in EC, and also in preadipocytes and adipocytes of white and brown fat depots. Furthermore, capillary sprouts from human adipose tissue, which have predominantly EC characteristics, are found to express Zfp423, a recently identified marker of preadipocyte determination. In response to PPARγ activation, endothelial characteristics of sprouting cells are progressively lost, and cells form structurally and biochemically defined adipocytes. Together these data support an endothelial origin of murine and human adipocytes, suggesting a model for how adipogenesis and angiogenesis are coordinated during adipose tissue expansion.
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影响因子：
37.8
作者：
Gealekman O;Guseva N;Hartigan C;Apotheker S;Gorgoglione M;Gurav K;Tran KV;Straubhaar J;Nicoloro S;Czech MP;Thompson M;Perugini RA;Corvera S
通讯作者：
Corvera S
影响因子：
64.5
作者：
Rodeheffer, Matthew S.;Birsoy, Kivanc;Friedman, Jeffrey M.
通讯作者：
Friedman, Jeffrey M.
影响因子：
15.9
作者：
Crossno, Joseph T., Jr.;Majka, Susan M.;Klemm, Dwight J.
通讯作者：
Klemm, Dwight J.
影响因子：
2.5
作者：
Alva, JA;Zovein, AC;Iruela-Arispe, ML
通讯作者：
Iruela-Arispe, ML
影响因子：
7.8
作者：
De Angelis, L;Berghella, L;Coletta, M;Lattanzi, L;Zanchi, M;Cusella-De Angelis, MG;Ponzetto, C;Cossu, G
通讯作者：
Cossu, G