Interaction of Saccharomyces boulardii with Salmonella enterica serovar Typhimurium protects mice and modifies T84 cell response to the infection.
Interaction of Saccharomyces boulardii with Salmonella enterica serovar Typhimurium protects mice and modifies T84 cell response to the infection.
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DOI：
10.1371/journal.pone.0008925
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发表时间：
2010-01-27
期刊：
影响因子：
3.7
通讯作者：
Czerucka D
中科院分区：
文献类型：
--
作者：
Martins FS;Dalmasso G;Arantes RM;Doye A;Lemichez E;Lagadec P;Imbert V;Peyron JF;Rampal P;Nicoli JR;Czerucka D
Salmonella pathogenesis engages host cells in two-way biochemical interactions: phagocytosis of bacteria by recruitment of cellular small GTP-binding proteins induced by the bacteria, and by triggering a pro-inflammatory response through activation of MAPKs and nuclear translocation of NF-κB. Worldwide interest in the use of functional foods containing probiotic bacteria for health promotion and disease prevention has increased significantly.布拉氏酵母菌是一种非致病性酵母，用作感染性腹泻的益生菌。 In this study, we reported that S. boulardii (Sb) protected mice from Salmonella enterica serovar Typhimurium (ST)-induced death and prevented bacterial translocation to the liver. At a molecular level, using T84 human colorectal cancer cells, we demonstrate that incubation with Sb before infection totally abolished Salmonella invasion.这与 Rac1 激活的减少相关。 Sb 保留了 T84 屏障功能并减少 ST 诱导的 IL-8 合成。 This anti-inflammatory effect was correlated with an inhibitory effect of Sb on ST-induced activation of the MAPKs ERK1/2, p38 and JNK as well as on activation of NF-κB. Electron and confocal microscopy experiments showed an adhesion of bacteria to yeast cells, which could represent one of the mechanisms by which Sb exerts its protective effects. Sb shows modulating effects on permeability, inflammation, and signal transduction pathway in T84 cells infected by ST and an in vivo protective effect against ST infection.目前的结果还表明，锑改变了沙门氏菌的侵袭特性。
Salmonella pathogenesis engages host cells in two-way biochemical interactions: phagocytosis of bacteria by recruitment of cellular small GTP-binding proteins induced by the bacteria, and by triggering a pro-inflammatory response through activation of MAPKs and nuclear translocation of NF-κB. Worldwide interest in the use of functional foods containing probiotic bacteria for health promotion and disease prevention has increased significantly. Saccharomyces boulardii is a non-pathogenic yeast used as a probiotic in infectious diarrhea. In this study, we reported that S. boulardii (Sb) protected mice from Salmonella enterica serovar Typhimurium (ST)-induced death and prevented bacterial translocation to the liver. At a molecular level, using T84 human colorectal cancer cells, we demonstrate that incubation with Sb before infection totally abolished Salmonella invasion. This correlates with a decrease of activation of Rac1. Sb preserved T84 barrier function and decreased ST-induced IL-8 synthesis. This anti-inflammatory effect was correlated with an inhibitory effect of Sb on ST-induced activation of the MAPKs ERK1/2, p38 and JNK as well as on activation of NF-κB. Electron and confocal microscopy experiments showed an adhesion of bacteria to yeast cells, which could represent one of the mechanisms by which Sb exerts its protective effects. Sb shows modulating effects on permeability, inflammation, and signal transduction pathway in T84 cells infected by ST and an in vivo protective effect against ST infection. The present results also demonstrate that Sb modifies invasive properties of Salmonella.
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DOI：
10.1016/j.bbrc.2006.02.080
发表时间：
2006-04-28
影响因子：
3.1
作者：
Sougioultzis, S;Simeonidis, S;Kelly, CP
通讯作者：
Kelly, CP
影响因子：
29.4
作者：
POTHOULAKIS, C;KELLY, CP;LAMONT, JT
通讯作者：
LAMONT, JT
影响因子：
3
作者：
Martins, Flaviano S.;Rodrigues, Ana Cristina P.;Nicoli, Jacques R.
通讯作者：
Nicoli, Jacques R.
影响因子：
7.8
作者：
McCormick, B A;Colgan, S P;Delp-Archer, C;Miller, S I;Madara, J L
通讯作者：
Madara, J L
影响因子：
3.1
作者：
Castagliuolo, I;LaMont, JM;Pothoulakis, C
通讯作者：
Pothoulakis, C