Copper Ions Facilitate the Conjugative Transfer of SXT/R391 Integrative and Conjugative Element Across Bacterial Genera.
Copper Ions Facilitate the Conjugative Transfer of SXT/R391 Integrative and Conjugative Element Across Bacterial Genera.
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铜离子促进 SXT/R391 整合和接合元件在细菌属间的接合转移。
DOI：
10.3389/fmicb.2020.616792
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发表时间：
2020
影响因子：
5.2
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Song Z;Zuo L;Li C;Tian Y;Wang H
铜可以长期稳定地存在于环境中。铜离子(Cu2+)除了能诱导细菌产生抗药性外，还能促进质粒DNA的水平转移。然而，Cu2+是否以及如何加速SXT/R391整合和共轭元件(ICE)的共轭转移仍是一个未知数。本研究以携带21个抗生素耐药基因(ARGs)的SXT/R391ICE的奇异变形杆菌CHSC1905为供体，以大肠杆菌EC600为受体。在亚抑制浓度和环境相关浓度(1-10μ/L)下，Cu2+显著促进了SXT/R391ICE在细菌属间的接合转移(P<；005)。表型试验和全基因组测序的联合分析表明，活性氧(ROS)的产生和细胞膜的通透性在SXT/R391ICE的接合转移增强中起关键作用。此外，当Cu2+浓度为5μ/L时，细胞黏附和三磷酸腺苷合成相关基因的表达也显著上调。本研究阐明了Cu2+促进SXT/R391ICE接合转移的可能机制，揭示了Cu2+对SXT/R391ICE介导的ArGs水平转移的潜在风险。
Copper can persist stably in the environment for prolonged periods. Except for inducing antibiotic resistance in bacteria, copper ions (Cu2+) can facilitate the horizontal transfer of plasmid DNA. However, whether and how Cu2+ can accelerate the conjugative transfer of SXT/R391 integrative and conjugative element (ICE) is still largely unknown. In this study, Proteus mirabilis ChSC1905, harboring an SXT/R391 ICE that carried 21 antibiotic resistance genes (ARGs), was used as a donor, and Escherichia coli EC600 was used as a recipient. Cu2+, at subinhibitory and environmentally relevant concentrations (1–10 μmol/L), significantly accelerated the conjugative transfer of SXT/R391 ICE across bacterial genera (from P. mirabilis to E. coli) (p < 0.05). The combined analyses of phenotypic tests and genome-wide sequencing indicated that reactive oxygen species (ROS) production and cell membrane permeability were critical in the enhanced conjugative transfer of SXT/R391 ICE. Furthermore, the expression of genes related to cell adhesion and ATP synthesis was also significantly upregulated on exposure to Cu2+ at a concentration of 5 μmol/L. This study clarified the potential mechanisms of Cu2+ to promote the conjugative transfer of SXT/R391 ICE, revealing the potential risk imposed by Cu2+ on the horizontal transfer of SXT/R391 ICE-mediated ARGs.
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