Existence of prophenoloxidase in wing discs: a source of plasma prophenoloxidase in the silkworm, Bombyx mori.
Existence of prophenoloxidase in wing discs: a source of plasma prophenoloxidase in the silkworm, Bombyx mori.
复制标题
DOI：
10.1371/journal.pone.0041416
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Ling E
中科院分区：
文献类型：
--
作者：
Diao Y;Lu A;Yang B;Hu W;Peng Q;Ling QZ;Beerntsen BT;Söderhäll K;Ling E
在昆虫中，血细胞被认为是血浆酚氧化酶原（PPO）的唯一来源。PPO也存在于与家蚕翅盘相连的造血器官的血细胞中。目前尚不清楚是否有其他细胞或组织可以产生PPO并将其释放到血淋巴中。在本研究中，我们使用家蚕作为模型来探索这种可能性。通过组织染色和生化分析，我们发现翼盘含有PPO，可以在体外释放到培养基中。原位分析表明，在翅盘腔中的一些细胞具有PPO 1和PPO 2 mRNA。我们的结论是造血器官可能错误地将血细胞释放到翅盘中，因为它们通过许多管道连接，如以前的文章所述。在翅盘中，浸润的血细胞可能通过细胞裂解产生和释放PPO，然后将PPO运输到血淋巴中。因此，这可能是家蚕血浆PPO的另一个来源：一些来自造血器官的浸润血细胞通过翅盘释放PPO。
In insects, hemocytes are considered as the only source of plasma prophenoloxidase (PPO). PPO also exists in the hemocytes of the hematopoietic organ that is connected to the wing disc of Bombyx mori. It is unknown whether there are other cells or tissues that can produce PPO and release it into the hemolymph besides circulating hemocytes. In this study, we use the silkworm as a model to explore this possibility. Through tissue staining and biochemical assays, we found that wing discs contain PPO that can be released into the culture medium in vitro. An in situ assay showed that some cells in the cavity of wing discs have PPO1 and PPO2 mRNA. We conclude that the hematopoietic organ may wrongly release hemocytes into wing discs since they are connected through many tubes as repost in previous paper. In wing discs, the infiltrating hemocytes produce and release PPO probably through cell lysis and the PPO is later transported into hemolymph. Therefore, this might be another source of plasma PPO in the silkworm: some infiltrated hemocytes sourced from the hematopoietic organ release PPO via wing discs.
登录
查看更多内容
昆虫后肠先天免疫和通过黑色化调节粪便微生物群
影响因子：
4.8
作者：
Shao, Qimiao;Yang, Bing;Ling, Erjun
通讯作者：
Ling, Erjun
影响因子：
0.9
作者：
Han, SS;Lee, MH;Yoe, SM
通讯作者：
Yoe, SM
影响因子：
5.8
作者：
Hillyer, JF;Schmidt, SL;Christensen, BM
通讯作者：
Christensen, BM
影响因子：
2.9
作者：
Ling, E;Shirai, K;Watanabe, H
通讯作者：
Watanabe, H
影响因子：
4.4
作者：
Dittmer, Neal T.;Hiromasa, Yasuaki;Kanost, Michael R.
通讯作者：
Kanost, Michael R.