Analysis of plant-derived miRNAs in animal small RNA datasets.
Analysis of plant-derived miRNAs in animal small RNA datasets.
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DOI：
10.1186/1471-2164-13-381
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发表时间：
2012-08-08
期刊：
影响因子：
4.4
通讯作者：
Heck G
中科院分区：
文献类型：
--
作者：
Zhang Y;Wiggins BE;Lawrence C;Petrick J;Ivashuta S;Heck G
植物含有大量来自不同sRNA生物发生途径的小rna （sRNAs）。其中许多srna在植物中起调节作用。先前的分析表明，玉米、水稻和大豆种子中的许多sRNAs与动物基因具有高度的序列相似性。然而，外源性RNA被认为在许多动物的胃肠道中是不稳定的，因此限制了食用膳食RNA的任何不利影响的可能性。最近的一篇论文报道，在动物血浆和血清中检测到假定的植物mirna，可能是通过摄入获得的，并且可能对食用生物产生功能影响。为了解决这种现象有多普遍的问题，我们在哺乳动物、鸡和昆虫的各种组织的公共sRNA数据集中搜索了植物miRNAs序列。我们的分析显示，植物mirna存在于动物sRNA数据集中，并且miR168的代表性非常高。此外，在大多数数据集中，包括在双科植物上饲养的两种昆虫的数据集，所有或几乎所有（bb0 96%）的miR168序列都是单子叶衍生的。为了研究植物来源的mirna（包括miR168）是否可以在昆虫体内系统地积累和移动，我们对包括玉米根虫在内的三种昆虫进行了昆虫摄食研究，这些昆虫已被证明对植物产生的长双链rna有反应。我们的分析表明，在动物sRNA数据集中观察到的植物mirna可能起源于测序过程，并且通过饮食暴露积累的植物mirna在动物中并不普遍。
Plants contain significant quantities of small RNAs (sRNAs) derived from various sRNA biogenesis pathways. Many of these sRNAs play regulatory roles in plants. Previous analysis revealed that numerous sRNAs in corn, rice and soybean seeds have high sequence similarity to animal genes. However, exogenous RNA is considered to be unstable within the gastrointestinal tract of many animals, thus limiting potential for any adverse effects from consumption of dietary RNA. A recent paper reported that putative plant miRNAs were detected in animal plasma and serum, presumably acquired through ingestion, and may have a functional impact in the consuming organisms. To address the question of how common this phenomenon could be, we searched for plant miRNAs sequences in public sRNA datasets from various tissues of mammals, chicken and insects. Our analyses revealed that plant miRNAs were present in the animal sRNA datasets, and significantly miR168 was extremely over-represented. Furthermore, all or nearly all (>96%) miR168 sequences were monocot derived for most datasets, including datasets for two insects reared on dicot plants in their respective experiments. To investigate if plant-derived miRNAs, including miR168, could accumulate and move systemically in insects, we conducted insect feeding studies for three insects including corn rootworm, which has been shown to be responsive to plant-produced long double-stranded RNAs. Our analyses suggest that the observed plant miRNAs in animal sRNA datasets can originate in the process of sequencing, and that accumulation of plant miRNAs via dietary exposure is not universal in animals.
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影响因子：
4.4
作者：
Chen C;Ai H;Ren J;Li W;Li P;Qiao R;Ouyang J;Yang M;Ma J;Huang L
通讯作者：
Huang L
影响因子：
14.9
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通讯作者：
Griffiths-Jones S
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4.4
作者：
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通讯作者：
Jaubert-Possamai S
影响因子：
4.3
作者：
Ivashuta, Sergey I.;Petrick, Jay S.;Roberts, James K.
通讯作者：
Roberts, James K.
影响因子：
6.6
作者：
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