Bioinformatic prediction, deep sequencing of microRNAs and expression analysis during phenotypic plasticity in the pea aphid, Acyrthosiphon pisum.
Bioinformatic prediction, deep sequencing of microRNAs and expression analysis during phenotypic plasticity in the pea aphid, Acyrthosiphon pisum.
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DOI：
10.1186/1471-2164-11-281
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发表时间：
2010-05-05
期刊：
影响因子：
4.4
通讯作者：
Jaubert-Possamai S
中科院分区：
文献类型：
--
作者：
Legeai F;Rizk G;Walsh T;Edwards O;Gordon K;Lavenier D;Leterme N;Méreau A;Nicolas J;Tagu D;Jaubert-Possamai S
真核生物的转录后调控可以通过microRNA （mirna）介导的基因沉默来实现。mirna是一种小的（18-25个核苷酸）非编码rna，在真核生物的基因表达调控中起着至关重要的作用。在昆虫中，mirna已被证明参与多种机制，如胚胎发育、组织分化、变态或昼夜节律。昆虫mirna已在鞘翅目、双翅目、膜翅目、鳞翅目和直翅目5个目的不同种中被鉴定出来。我们对豌豆蚜虫Acyrthosiphon pisum的基因组进行了高通量Solexa测序和生物信息学分析，以鉴定来自半翅类昆虫的第一个mirna。通过结合这些方法，我们鉴定了149个mirna，其中包括55个保守的mirna和94个新的mirna。此外，我们通过分析miRNAs在繁殖模式切换中的表达，研究了这些miRNAs在豌豆蚜虫不同替代形态中的调控。豌豆蚜虫的microRNA序列已经发布到miRBase: http://microrna.sanger.ac.uk/sequences/我们的研究已经确定了可能参与蚜虫生殖多表型的调节因子，并为进一步的功能分析开辟了新的途径。
Post-transcriptional regulation in eukaryotes can be operated through microRNA (miRNAs) mediated gene silencing. MiRNAs are small (18-25 nucleotides) non-coding RNAs that play crucial role in regulation of gene expression in eukaryotes. In insects, miRNAs have been shown to be involved in multiple mechanisms such as embryonic development, tissue differentiation, metamorphosis or circadian rhythm. Insect miRNAs have been identified in different species belonging to five orders: Coleoptera, Diptera, Hymenoptera, Lepidoptera and Orthoptera. We developed high throughput Solexa sequencing and bioinformatic analyses of the genome of the pea aphid Acyrthosiphon pisum in order to identify the first miRNAs from a hemipteran insect. By combining these methods we identified 149 miRNAs including 55 conserved and 94 new miRNAs. Moreover, we investigated the regulation of these miRNAs in different alternative morphs of the pea aphid by analysing the expression of miRNAs across the switch of reproduction mode. Pea aphid microRNA sequences have been posted to miRBase: http://microrna.sanger.ac.uk/sequences/ Our study has identified candidates as putative regulators involved in reproductive polyphenism in aphids and opens new avenues for further functional analyses.
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影响因子：
1.8
作者：
HOFACKER, IL;FONTANA, W;SCHUSTER, P
通讯作者：
SCHUSTER, P
影响因子：
30.8
作者：
Bentwich, I;Avniel, A;Bentwich, Z
通讯作者：
Bentwich, Z
Mirbase：MicroRNA基因组学的工具。
影响因子：
14.9
作者：
Griffiths-Jones, Sam;Saini, Harpreet Kaur;van Dongen, Stijn;Enright, Anton J.
通讯作者：
Enright, Anton J.
影响因子：
16.8
作者：
Okamura, Katsutomo;Phillips, Michael D.;Lai, Eric C.
通讯作者：
Lai, Eric C.
影响因子：
56.9
作者：
Reinhart, BJ;Bartel, DP
通讯作者：
Bartel, DP