Overexpression of Tyrosine hydroxylase and Dopa decarboxylase associated with pupal melanization in Spodoptera exigua.
Overexpression of Tyrosine hydroxylase and Dopa decarboxylase associated with pupal melanization in Spodoptera exigua.
复制标题
酪氨酸羟化酶和多巴脱羧酶的过度表达与甜菜夜蛾蛹黑色化相关。
DOI：
10.1038/srep11273
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发表时间：
2015-06-18
影响因子：
4.6
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Liu S;Wang M;Li X
黑变现象在许多物种中都有发现，但涉及的分子机制在很大程度上仍然难以捉摸。本研究对甜菜夜蛾蛹黑化的分子机制进行了研究。克隆了黑色素生物合成途径中的两个关键酶酪氨酸羟化酶（TH）和多巴脱羧酶（DDC）的cDNA全长序列，并比较了它们在黑色素发育过程中的表达规律。甜菜野生型（SEW）和黑素突变体（SEM）菌株。TH和DDC的蛋白质序列在两株菌中没有发现氨基酸的变化。与SEW品系相比，DDC和TH在SEM品系前蛹后期和0 h蛹的体壁中均显著过表达。用含DDC抑制剂L-α-Methyl-DOPA 1 mg/g和TH抑制剂3-iodo-tyrosine（3-IT）0.75mg/g的饲料饲喂5龄幼虫，分别有20%和68.2%的蛹具有部分拯救表型和完全拯救表型。这些结果表明，TH和DDC的过度表达参与了蛹的黑化过程。exigua.
Melanism has been found in a wide range of species, but the molecular mechanisms involved remain largely elusive. In this study, we studied the molecular mechanisms of the pupal melanism in Spodoptera exigua. The full length cDNA sequences of tyrosine hydroxylase (TH) and dopa decarboxylase (DDC), two key enzymes in the biosynthesis pathway of melanin, were cloned, and their temporal expression patterns in the integument were compared during the larval-pupal metamorphosis process of the S. exigua wild type (SEW) and melanic mutant (SEM) strains. No amino acid change in the protein sequence of TH and DDC was found between the two strains. Both DDC and TH were significantly over-expressed in the integument of the SEM strain at late-prepupa and 0 h pupa, respectively, compared with those of the SEW strain. Feeding 5th instar larvae of SEM with diets incorporated with 1 mg/g of the DDC inhibitor L-α-Methyl-DOPA and 0.75 mg/g of the TH inhibitor 3-iodo-tyrosine (3-IT) resulted in 20% pupae with partially-rescued phenotype and 68.2% of pupae with partially- or fully-rescued phenotype, respectively. These results indicate that overexpressions of TH and DDC are involved in the pupal melanization of S. exigua.
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影响因子：
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