Plasmodium malariae in the Colombian Amazon region: you don't diagnose what you don't suspect.
Plasmodium malariae in the Colombian Amazon region: you don't diagnose what you don't suspect.
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DOI：
10.1186/s12936-016-1629-3
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发表时间：
2016-11-29
期刊：
影响因子：
3
通讯作者：
Patarroyo MA
中科院分区：
文献类型：
--
作者：
Niño CH;Cubides JR;Camargo-Ayala PA;Rodríguez-Celis CA;Quiñones T;Cortés-Castillo MT;Sánchez-Suárez L;Sánchez R;Patarroyo ME;Patarroyo MA
疟疾是一个世界性的公共卫生问题;来自疟原虫属的寄生虫。是这种疾病的病原体这种寄生虫主要通过显微镜技术进行诊断。然而，这些具有有限的灵敏度。许多无症状感染是亚显微镜下的，只能通过分子方法检测。这项研究的目的是比较巢式PCR结果，通过显微镜诊断疟疾，并提出有关疟疾在哥伦比亚的亚马逊省的流行病学数据。共1392血液样本（静脉穿刺）从症状患者在哥伦比亚的亚马逊省进行了分析，在平行厚血涂片（TBS）测试和巢式PCR确定疟原虫属。感染和鉴定感染物种，例如间日疟原虫、三日疟原虫和/或恶性疟原虫。描述性统计用于比较两种试验的结果，包括疾病检测、感染物种分型及其在研究区域的流行率。以显微镜检测为金标准，通过统计学指标评价PCR诊断性能。本研究揭示了两种诊断测试之间的巨大差异，以及从分子角度表明三日疟原虫的高患病率。这与以前在哥伦比亚这一地区进行的研究（通常基于TBS测试）有很大不同，这表明通过常规技术进行诊断可能会导致低估某些疟原虫的流行率。在这个地区有很高的流通量。目前的研究结果强调，需要修改国家疟疾监测计划，更有效的战略，在流行地区检测这种疾病。还强调了聚合酶链反应作为低寄生虫血症或混合感染病例的备用检测的重要性。本文的在线版本（doi：10.1186/s12936-016-1629-3）包含补充材料，可供授权用户使用。
Malaria is a worldwide public health problem; parasites from the genus Plasmodium spp. are the aetiological agent of this disease. The parasite is mainly diagnosed by microscope-based techniques. However, these have limited sensitivity. Many asymptomatic infections are sub-microscopic and can only be detected by molecular methods. This study was aimed at comparing nested PCR results to those obtained by microscope for diagnosing malaria and to present epidemiological data regarding malaria in Colombia’s Amazon department. A total of 1392 blood samples (taken by venepuncture) from symptomatic patients in Colombia’s Amazon department were analysed in parallel by thick blood smear (TBS) test and nested PCR for determining Plasmodium spp. infection and identifying infecting species, such as Plasmodium vivax, Plasmodium malariae and/or Plasmodium falciparum. Descriptive statistics were used for comparing the results from both tests regarding detection of the disease, typing infecting species and their prevalence in the study region. Bearing the microscope assay in mind as gold standard, PCR diagnosis performance was evaluated by statistical indicators. The present study revealed great differences between both diagnostic tests, as well as suggesting high P. malariae prevalence from a molecular perspective. This differed profoundly from previous studies in this region of Colombia, usually based on the TBS test, suggesting that diagnosis by conventional techniques could lead to underestimating the prevalence of certain Plasmodium spp. having high circulation in this area. The present results highlight the need for modifying state malaria surveillance schemes for more efficient strategies regarding the detection of this disease in endemic areas. The importance of PCR as a back-up test in cases of low parasitaemia or mixed infection is also highlighted. The online version of this article (doi:10.1186/s12936-016-1629-3) contains supplementary material, which is available to authorized users.
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DOI：
10.1093/infdis/jit184
发表时间：
2013-08-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Hopkins H;González IJ;Polley SD;Angutoko P;Ategeka J;Asiimwe C;Agaba B;Kyabayinze DJ;Sutherland CJ;Perkins MD;Bell D
通讯作者：
Bell D
影响因子：
3
作者：
Andrade BB;Reis-Filho A;Barros AM;Souza-Neto SM;Nogueira LL;Fukutani KF;Camargo EP;Camargo LM;Barral A;Duarte A;Barral-Netto M
通讯作者：
Barral-Netto M
影响因子：
11.8
作者：
Krishna S;Bharti PK;Chandel HS;Ahmad A;Kumar R;Singh PP;Singh MP;Singh N
通讯作者：
Singh N
影响因子：
1.6
作者：
Dhiman, Sunil;Goswami, Diganta;Veer, Vijay
通讯作者：
Veer, Vijay
影响因子：
3
作者：
Britton S;Cheng Q;McCarthy JS
通讯作者：
McCarthy JS