Low-diversity bacterial community in the gut of the fruitfly Drosophila melanogaster.
Low-diversity bacterial community in the gut of the fruitfly Drosophila melanogaster.
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DOI：
10.1111/j.1462-2920.2011.02511.x
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发表时间：
2011-07
影响因子：
5.1
通讯作者：
Douglas AE
中科院分区：
文献类型：
--
作者：
Wong CN;Ng P;Douglas AE
通过 16S rRNA 基因扩增子的 454 焦磷酸测序对果蝇不同生命阶段的细菌进行定量。序列读数主要由 5 个操作分类单位 (OTU) 组成，序列同一性≤ 97%，可归属于醋杆菌、热带曲霉、短乳杆菌、果糖乳杆菌和植物乳杆菌。饱和稀疏曲线和物种丰富度指数表明采样（每个样本 85 000–159 000 个读数）是全面的。平行诊断 PCR 检测显示，个体昆虫和三种黑腹果蝇菌株中的五种细菌种类的互补性仅存在微小差异。其他肠道相关细菌包括 6 个 OTU，其与之前报道的序列的 %ID 较低，这提高了它们代表醋杆菌属和乳杆菌属中新分类群的可能性。确定了最丰富的物种的发育变化，从年轻成虫的 L. fructivorans 到老年成虫的 A. pomorum；肠道氧张力或免疫系统功能的变化可能是造成这种影响的原因。宿主免疫反应和干扰也可能导致果蝇肠道栖息地细菌多样性低。
The bacteria in the fruitfly Drosophila melanogaster of different life stages was quantified by 454 pyrosequencing of 16S rRNA gene amplicons. The sequence reads were dominated by 5 operational taxonomic units (OTUs) at ≤ 97% sequence identity that could be assigned to Acetobacter pomorum, A. tropicalis, Lactobacillus brevis, L. fructivorans and L. plantarum. The saturated rarefaction curves and species richness indices indicated that the sampling (85 000–159 000 reads per sample) was comprehensive. Parallel diagnostic PCR assays revealed only minor variation in the complement of the five bacterial species across individual insects and three D. melanogaster strains. Other gut-associated bacteria included 6 OTUs with low %ID to previously reported sequences, raising the possibility that they represent novel taxa within the genera Acetobacter and Lactobacillus. A developmental change in the most abundant species, from L. fructivorans in young adults to A. pomorum in aged adults was identified; changes in gut oxygen tension or immune system function might account for this effect. Host immune responses and disturbance may also contribute to the low bacterial diversity in the Drosophila gut habitat.
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影响因子：
12.3
作者：
Huse SM;Huber JA;Morrison HG;Sogin ML;Welch DM
通讯作者：
Welch DM
影响因子：
3.7
作者：
Garren M;Raymundo L;Guest J;Harvell CD;Azam F
通讯作者：
Azam F
影响因子：
3.4
作者：
BAKULA, M
通讯作者：
BAKULA, M
影响因子：
4.4
作者：
Ashelford, Kevin E.;Chuzhanova, Nadia A.;Weightman, Andrew J.
通讯作者：
Weightman, Andrew J.
影响因子：
3.4
作者：
Gruenwald, S.;Pilhofer, M.;Hoell, W.
通讯作者：
Hoell, W.