A high-throughput method to deliver targeted optogenetic stimulation to moving C. elegans populations.
A high-throughput method to deliver targeted optogenetic stimulation to moving C. elegans populations.
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DOI：
10.1371/journal.pbio.3001524
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发表时间：
2022-01
期刊：
影响因子：
9.8
通讯作者：
Leifer AM
中科院分区：
文献类型：
--
作者：
Liu M;Kumar S;Sharma AK;Leifer AM
我们提出了一种高通量光遗传学照明系统，能够同时闭环光传递到移动的秀丽隐杆线虫种群中的指定目标。该仪器解决了三个技术挑战：它为动物身体的指定区域（如头部或尾部）提供有针对性的照明;它根据动物的行为自动提供刺激;它通过同时瞄准许多动物来实现高通量。该仪器用于光遗传学探测动物对前部和后部轻柔触觉受体神经元中的竞争性机械感觉刺激的行为反应。测量了来自一系列前后强度组合的超过43，418个刺激事件的反应。该动物的概率向前冲刺响应于mechanosensory刺激取决于前和后刺激强度，而逆转的概率主要取决于前刺激强度。我们还探讨了动物的反应，机械感官刺激的开始转向，一个相对罕见的行为事件，自动提供刺激时，动物开始转向。使用这种闭环方法，超过9，700刺激事件在转向开始期间以每蠕虫小时9.2事件的速率传递，与以前的调查相比，吞吐量增加了25倍以上。这些测量验证了更大的统计功率以前的研究结果，转向行为门机械感应诱发逆转。与以前的方法相比，目前的系统为特定的身体区域或行为提供了靶向的光遗传学刺激，其吞吐量增加了许多倍，以更好地约束感觉运动处理的定量模型。这项研究提出了一种新的针对秀丽隐杆线虫的靶向照明方法，允许将光遗传学刺激同时传递到许多动物的特定身体部位，由动物的行为自动触发。
We present a high-throughput optogenetic illumination system capable of simultaneous closed-loop light delivery to specified targets in populations of moving Caenorhabditis elegans. The instrument addresses three technical challenges: It delivers targeted illumination to specified regions of the animal’s body such as its head or tail; it automatically delivers stimuli triggered upon the animal’s behavior; and it achieves high throughput by targeting many animals simultaneously. The instrument was used to optogenetically probe the animal’s behavioral response to competing mechanosensory stimuli in the the anterior and posterior gentle touch receptor neurons. Responses to more than 43,418 stimulus events from a range of anterior–posterior intensity combinations were measured. The animal’s probability of sprinting forward in response to a mechanosensory stimulus depended on both the anterior and posterior stimulation intensity, while the probability of reversing depended primarily on the anterior stimulation intensity. We also probed the animal’s response to mechanosensory stimulation during the onset of turning, a relatively rare behavioral event, by delivering stimuli automatically when the animal began to turn. Using this closed-loop approach, over 9,700 stimulus events were delivered during turning onset at a rate of 9.2 events per worm hour, a greater than 25-fold increase in throughput compared to previous investigations. These measurements validate with greater statistical power previous findings that turning acts to gate mechanosensory evoked reversals. Compared to previous approaches, the current system offers targeted optogenetic stimulation to specific body regions or behaviors with many fold increases in throughput to better constrain quantitative models of sensorimotor processing. This study resents a new targeted illumination method for the nematode Caenorhabditis elegans, allowing delivery of optogenetic stimulation to specific body parts of many animals at once, automatically triggered by the animals’ behavior.
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影响因子：
9.8
作者：
Donnelly JL;Clark CM;Leifer AM;Pirri JK;Haburcak M;Francis MM;Samuel AD;Alkema MJ
通讯作者：
Alkema MJ
影响因子：
13.9
作者：
Fenno L;Yizhar O;Deisseroth K
通讯作者：
Deisseroth K
影响因子：
48
作者：
Gershow, Marc;Berck, Matthew;Mathew, Dennis;Luo, Linjiao;Kane, Elizabeth A.;Carlson, John R.;Samuel, Aravinthan D. T.
通讯作者：
Samuel, Aravinthan D. T.
影响因子：
7.7
作者：
Cande J;Namiki S;Qiu J;Korff W;Card GM;Shaevitz JW;Stern DL;Berman GJ
通讯作者：
Berman GJ
影响因子：
2.7
作者：
CHALFIE, M;SULSTON, J
通讯作者：
SULSTON, J