Effects of abscisic acid on ethylene biosynthesis and perception in Hibiscus rosa-sinensis L. flower development.
Effects of abscisic acid on ethylene biosynthesis and perception in Hibiscus rosa-sinensis L. flower development.
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DOI：
10.1093/jxb/err218
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发表时间：
2011-11
影响因子：
6.9
通讯作者：
Serra G
中科院分区：
文献类型：
--
作者：
Trivellini A;Ferrante A;Vernieri P;Serra G
研究了乙烯与脱落酸(ABA)的复杂关系对芙蓉花发育和衰老的影响。分离了乙烯生物合成(HrsACS和HrsACO)和受体(HrsETR和HrsERS)基因，并研究了它们在花瓣、花柱柱头+雄蕊和子房三种不同的花组织中的表达。用0.1 mM 1-氨基环丙烷-1-羧酸溶液、500 nL L−1-甲基环丙烯(1- mM)和0.1 mM脱落酸溶液处理。ACC和1-MCP处理证实了木槿花的衰老依赖于乙烯，外源ABA处理表明ABA可能在这一过程中起作用。1-甲基环丙烯抑制花瓣卷曲，9 h后开放花脱落酸含量下降，1-甲基环丙烯处理的花瓣和花柱柱头+雄蕊的脱落酸含量在1-6 h之间先升高，6 h和9 h后显著促进花的衰老和乙烯的产生，尤其是花柱柱头+雄蕊。这些花组织中的乙烯释放与乙烯生物合成和受体基因表达的诱导呈正相关。最后，ABA对所有花组织中的乙烯生物合成途径和组织敏感性产生了负面影响。外源ABA处理降低了HrsACS、HrsACO、HrsETR和HrsERS的转录丰度。这项研究强调了当外源应用衰老抑制剂或促进剂时，ABA在生理和分子水平上对乙烯生物合成和感知机制的调节作用。
The effect of the complex relationship between ethylene and abscisic acid (ABA) on flower development and senescence in Hibiscus rosa-sinensis L. was investigated. Ethylene biosynthetic (HrsACS and HrsACO) and receptor (HrsETR and HrsERS) genes were isolated and their expression evaluated in three different floral tissues (petals, style–stigma plus stamens, and ovaries) of detached buds and open flowers. This was achieved through treatment with 0.1 mM 1-aminocyclopropane-1-carboxylic acid (ACC) solution, 500 nl l−1 methylcyclopropene (1-MCP), and 0.1 mM ABA solution. Treatment with ACC and 1-MCP confirmed that flower senescence in hibiscus is ethylene dependent, and treatment with exogenous ABA suggested that ABA may play a role in this process. The 1-MCP impeded petal in-rolling and decreased ABA content in detached open flowers after 9 h. This was preceded by an earlier and sequential increase in ABA content in 1-MCP-treated petals and style–stigma plus stamens between 1 h and 6 h. ACC treatment markedly accelerated flower senescence and increased ethylene production after 6 h and 9 h, particularly in style–stigma plus stamens. Ethylene evolution was positively correlated in these floral tissues with the induction of the gene expression of ethylene biosynthetic and receptor genes. Finally, ABA negatively affected the ethylene biosynthetic pathway and tissue sensitivity in all flower tissues. Transcript abundance of HrsACS, HrsACO, HrsETR, and HrsERS was reduced by exogenous ABA treatment. This research underlines the regulatory effect of ABA on the ethylene biosynthetic and perception machinery at a physiological and molecular level when inhibitors or promoters of senescence are exogenously applied.
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影响因子：
4.2
作者：
Aneja, M;Gianfagna, T;Ng, E
通讯作者：
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影响因子：
11.6
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通讯作者：
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作者：
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影响因子：
4.2
作者：
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作者：
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通讯作者：
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